Cell Cycle Arrest Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods Towards HeLa Cancer Cell

Aminah Dalimunthe, Poppy Anjelisa Zaitun Hasibuan, Denny Satria

Abstract

Background: Cervical cancer therapy with chemotherapeutic agents is limited because of drug resistance problem and toxic effect on normal tissue leads to immunosuppression and cardiotoxicity. Objective: This study was to investigated cell cycle arrest activity towards HeLa cell lines of Litsea cubeba Lour. heartwood alkaloid fraction. Methods: Litsea cubeba Lour. heartwood powder was extracted by maceration method with ethanol 96% and fractionated with n-hexane and chloroform at pH 3,7 and 9. The cytotoxic study was using MTT method and analysis cell cycle was using flow cytometry method. Results: The IC 50 of ethanol extract, n-hexane and chloroform fractions at pH 3,7 and 9 at were 156.24 ± 2.96; 67.23 ± 0.63; 175.92 ± 2.40; 52.46 ± 0.34; and 94.81 ± 2.16 µg/mL respectively. The chloroform fractions at pH 7 concentration 25 and 10 µg/mL were caused accumulation in G 2 -M phase (33.84 and 29.08%). Conclusions: The results reveal that Litsea cubeba Lour. heartwood alkaloid fraction provides effective as cell cycle arrest. Our further study is to assess the mechanism of alkaloid fraction in inhibit metastasis in cervical cancer.

Paper Citation

in Harvard Style

Dalimunthe A., Zaitun Hasibuan P. and Satria D. (2018). Cell Cycle Arrest Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods Towards HeLa Cancer Cell.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 174-177. DOI: 10.5220/0008359401740177

in Bibtex Style

@conference{bromo18,
author={Aminah Dalimunthe and Poppy Anjelisa Zaitun Hasibuan and Denny Satria},
title={Cell Cycle Arrest Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods Towards HeLa Cancer Cell},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={174-177},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008359401740177},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Cell Cycle Arrest Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods Towards HeLa Cancer Cell
SN - 978-989-758-347-6
AU - Dalimunthe A.
AU - Zaitun Hasibuan P.
AU - Satria D.
PY - 2018
SP - 174
EP - 177
DO - 10.5220/0008359401740177