Antibacterial Activity of Methanol Extracts and Compounds of Wualae (Etlingera elatior) Fruits from Southeast Sulawesi-Indonesia

I. Sahidin, Syefira Salsabila, W. Wahyuni, M. Hajrul Malaka, Imran, Marianti A. Manggau

Abstract

Wualae (Tolakinese) or Etlingera elatior grows bulky in Southeast Sulawesi. The fruit of this plant is widely used as cooking spices and traditional medicine. Scientific studies of the fruit and its properties against certain diseases are still very limited. The aim of the article shares the chemical content of E. elatior fruits and its activity against various selected pathogenic bacteria. Isolation was performed by chromatographic methods, including Thin Layer Chromatography (TLC), Vacuum Liquid Chromatography (VLC), and Radial Chromatography (RC). Structure of the isolated compounds was elucidated by using spectroscopic techniques, i.e. IR and NMR-1D spectroscopy ( 1 H and 13 C-NMR) and comparing with similar data from the literature. The activity of the methanol extracts and the isolated compounds were evaluated against bacteria using the diffusion agar method. The tested bacteria included Escherichia coli ATCC 35218, Pseudomonas aeruginosa, Staphylococcus aureus ATCC 25923, Bacilus subtilis, Streptococcus mutans ATCC 25175 and Salmonella enteric. The result showed that two compounds have been isolated from E. elatior fruit, namely vanilic acid (1) and p-hydroxybenzoic acid (2). The compounds and crude extracts were most active against S. mutans. The data is a reference where the methanol extracts of E.elatior fruits can be developed into a mouthwash or toothpaste.

Paper Citation

in Harvard Style

Sahidin I., Salsabila S., Wahyuni W., Malaka M., Imran. and Manggau M. (2018). Antibacterial Activity of Methanol Extracts and Compounds of Wualae (Etlingera elatior) Fruits from Southeast Sulawesi-Indonesia.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 151-154. DOI: 10.5220/0008358901510154

in Bibtex Style

@conference{bromo18,
author={I. Sahidin and Syefira Salsabila and W. Wahyuni and M. Hajrul Malaka and Imran and Marianti A. Manggau},
title={Antibacterial Activity of Methanol Extracts and Compounds of Wualae (Etlingera elatior) Fruits from Southeast Sulawesi-Indonesia},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={151-154},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008358901510154},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Antibacterial Activity of Methanol Extracts and Compounds of Wualae (Etlingera elatior) Fruits from Southeast Sulawesi-Indonesia
SN - 978-989-758-347-6
AU - Sahidin I.
AU - Salsabila S.
AU - Wahyuni W.
AU - Malaka M.
AU - Imran.
AU - Manggau M.
PY - 2018
SP - 151
EP - 154
DO - 10.5220/0008358901510154