The Study of the Antibacterial Activity of Asam Gelugor (Garcinia atroviridis) Against Methecillin-resistant Staphylococcus Aureus (MRSA), Streptococcus Pneumoniae and Klebseilla Pneumoniae

May Florence Dela Cruz Bacayo, Nurul Shahira Sajali, Wong Charng Choon, Santosh Fattepur, Kiran Chanabasappa Nilugal, Jiyauddin Khan, Fadli Asmani, Eddy Yusuf

Abstract

BACKGROUND: The curative ability of the endemic plants for different disorders has been described by traditional medicine practitioners. Some herbs have been reported to have antibacterial activity such as Garcinia Atroviridis. OBJECTIVE: The antibacterial activity of Garcinia Atroviridis (Asam Gelugor) extracts was evaluated against Methicillin-resistant Staphylococus aureus, Streptococcus pneumoniae and Klebseilla pneumoniae METHOD: Asam Gelugor fruits (600 grams) were collected. The powder form were successively extracted with with 99.8 % of methanol. The extract was filtered and dried using the rotatory evaporator at a temperature not exceeding 50ºC. The antibacterial activity was determined by disc diffusion method for the zone of inhibition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The zone of inhibition were compared with that of ampicillin, vancomycin, and gentamicin antibiotic disc. RESULT: The test by using disc diffusion method shows highest inhibition against the Klebsiella pneumoniae (15.33 ± 1.53) in 100% extract. Based on the result for MIC, inhibition is at 50mg/ml until 0.05 mg/ml against MRSA, while the MIC is positive in all concentration of extract against Klebsiella pneumoniae but the MIC result is negative in all concentration of the extract against Streptococcus pneumoniae. MBC result showed that there is bacterial growth in 500mg/ml of extract against MRSA while no bacterial growth in extract against Klebsiella pneumonia, bacterial growth is positive in the extract against the Streptococcus pneumoniae. CONCLUSION: The results shows that Asam Gelugor (Garcinia Atroviridis) may serve to the development of a new antibacterial agent against these type of bacteria.

Paper Citation

in Harvard Style

Bacayo M., Sajali N., Choon W., Fattepur S., Nilugal K., Khan J., Asmani F. and Yusuf E. (2018). The Study of the Antibacterial Activity of Asam Gelugor (Garcinia atroviridis) Against Methecillin-resistant Staphylococcus Aureus (MRSA), Streptococcus Pneumoniae and Klebseilla Pneumoniae.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 118-123. DOI: 10.5220/0008358401180123

in Bibtex Style

@conference{bromo18,
author={May Florence Dela Cruz Bacayo and Nurul Shahira Sajali and Wong Charng Choon and Santosh Fattepur and Kiran Chanabasappa Nilugal and Jiyauddin Khan and Fadli Asmani and Eddy Yusuf},
title={The Study of the Antibacterial Activity of Asam Gelugor (Garcinia atroviridis) Against Methecillin-resistant Staphylococcus Aureus (MRSA), Streptococcus Pneumoniae and Klebseilla Pneumoniae},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={118-123},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008358401180123},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - The Study of the Antibacterial Activity of Asam Gelugor (Garcinia atroviridis) Against Methecillin-resistant Staphylococcus Aureus (MRSA), Streptococcus Pneumoniae and Klebseilla Pneumoniae
SN - 978-989-758-347-6
AU - Bacayo M.
AU - Sajali N.
AU - Choon W.
AU - Fattepur S.
AU - Nilugal K.
AU - Khan J.
AU - Asmani F.
AU - Yusuf E.
PY - 2018
SP - 118
EP - 123
DO - 10.5220/0008358401180123