A Comparative Study of the Antimicrobial Activity of Wild (Tualang)
Honey and Artificial Honey Against Methecillin-resistant
Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella
Pneumoniae
May Florence Dela Cruz Bacayo
1
*, Shazwan Fahmi Shafi
1
, Wong Charng Choon
1
, Santosh Fattepur
1
,
Kiran Chanabasappa Nilugal
1
, Jiyauddin Khan
1
, Fadli Asmani
1
, Eddy Yusuf
1
1
School of Pharmacy, Management and Science University, 40100 Shah Alam Malaysia
Keywords: Tualang honey, Artificial honey; Antimicrobial activity.
Abstract : Background: Antimicrobial agents are becoming less effective due to the emergence of antibiotic-resistant
microbes, thus the discovery of alternative antimicrobial agents are urgently needed. Honey is believed to
have antimicrobial activity and are used in traditional medicine as skin healing dressing for wound.
Objectives: To find an alternative substance as a substitute for the current antimicrobial agent for the three
strains of microbes and to make a comparison between the antimicrobial activity of the wild (Tualang)
honey and the artificial honey. Method: The 50 % (w/v) of honey were diluted using double dilution method
and are tested for its antimicrobial activity using disc diffusion method with Ampicillin as a standard
antibiotic for Streptococcus pneumoniae, Gentamycin for Klebsiella pneumonia and Vancomycin for
Methicillin-resistant Staphylococcus aurues as positive control. The MIC and MBC were determined.
Results: Based on the results obtained from the disc diffussion assay, it shows that wild (Tualang) honey has
antimicrobial activity at a concentration of 10 % (w/v) for MRSA and S. pneumoniae while at 25 % (w/v) for
K. pneumoniae. Meanwhile it need 100% (w/v) concentration of artificial honey to shows its antimicrobial
activity. Wild (Tualang) honey was recorded as the most potent honey against S. pneumoniae, in which a
dilution of 10% (w/v) was required to inhibit the growth and kill S. pneumoniae colony at 20% (w/v). The
concentration of 20% (w/v) was required to inhibit MRSA and 25% (w/v) to kill MRSA. Meanwhile, the
highest concentration were required to inhibit K.pneumonia at 50% (w/v) and no bactericidal effect were
recorded. Conclusion: The comparative study between Tualang honey and Artificial honey gives a
promising result that the Tualang honey has a highest antimicrobial activity against MRSA, Klebsiella
pneumoniae, Sterptococcus pneumoniae compared to the Artificial honey.
1 INTRODUCTION
Honey is a natural syrup which have a complex
function of physicochemical properties (color, flavor
and texture), mainly determined by their botanic and
geographic origins. Honey is a concentrated aqueous
solution of glucose (31%) and fructose (39%). It
contains free amino acids at a level of 1%, pollen
being one of their sources. Proline, which might
originate from bees, is the prevalent amino acid and
makes up 50–85% of the amino acid fraction (White,
1975). Organic acids are present in honey at low
concentrations (<0.5%) and it is related to the color,
flavor and physico-chemical properties of the honey,
such as pH, acidity, and electrical conductivity.
Organic acids chelate metals can synergistically
enhance the antioxidant action of phenolic
compounds (Gheldof, Wang & Engeseth, 2002).
Moreover, acetic acid and ethanol can be used as
fermentation indicators and formic acid as an
indicator for the treatment of Varroa infestation
(Calderone, 2000).
It was used as a wound treatment that are
non-responsive to conventional therapies, such as
diabetic ulcers, and wounds infected with
antibiotic-resistant bacteria. There were countless
studies that recount the antimicrobial activity of
honey against microbes. (Dunford C, Cooper R,
Molan P, et al, 200).
With the emergence of antibiotic-resistant microbial
strains, such as Methicillin-resistant S. aureus that
can cause difficult-to-treat wound infections, honey
has again caught the attention of medical researchers.
Scientists had first reported the ability of honey to
treat infections caused by microbes in the late 1800s,
Bacayo, M., Shafi, S., Choon, W., Fattepur, S., Nilugal, K., Khan, J., Asmani, F. and Yusuf, E.
A Comparative Study of the Antimicrobial Activity of Wild (Tualang) Honey and Artificial Honey Against Methecillin- resistant Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella
Pneumoniae.
DOI: 10.5220/0008358301110117
In Proceedings of BROMO Conference (BROMO 2018), pages 111-117
ISBN: 978-989-758-347-6
Copyright
c
2018 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved
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