A Comparative Study of the Antimicrobial Activity of Wild (Tualang) Honey and Artificial Honey Against Methecillin- resistant Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella Pneumoniae

May Florence Dela Cruz Bacayo, Shazwan Fahmi Shafi, Wong Charng Choon, Santosh Fattepur, Kiran Chanabasappa Nilugal, Jiyauddin Khan, Fadli Asmani, Eddy Yusuf

Abstract

Background: Antimicrobial agents are becoming less effective due to the emergence of antibiotic-resistant microbes, thus the discovery of alternative antimicrobial agents are urgently needed. Honey is believed to have antimicrobial activity and are used in traditional medicine as skin healing dressing for wound. Objectives: To find an alternative substance as a substitute for the current antimicrobial agent for the three strains of microbes and to make a comparison between the antimicrobial activity of the wild (Tualang) honey and the artificial honey. Method: The 50 % (w/v) of honey were diluted using double dilution method and are tested for its antimicrobial activity using disc diffusion method with Ampicillin as a standard antibiotic for Streptococcus pneumoniae, Gentamycin for Klebsiella pneumonia and Vancomycin for Methicillin-resistant Staphylococcus aurues as positive control. The MIC and MBC were determined. Results: Based on the results obtained from the disc diffussion assay, it shows that wild (Tualang) honey has antimicrobial activity at a concentration of 10 % (w/v) for MRSA and S. pneumoniae while at 25 % (w/v) for K. pneumoniae. Meanwhile it need 100% (w/v) concentration of artificial honey to shows its antimicrobial activity. Wild (Tualang) honey was recorded as the most potent honey against S. pneumoniae, in which a dilution of 10% (w/v) was required to inhibit the growth and kill S. pneumoniae colony at 20% (w/v). The concentration of 20% (w/v) was required to inhibit MRSA and 25% (w/v) to kill MRSA. Meanwhile, the highest concentration were required to inhibit K.pneumonia at 50% (w/v) and no bactericidal effect were recorded. Conclusion: The comparative study between Tualang honey and Artificial honey gives a promising result that the Tualang honey has a highest antimicrobial activity against MRSA, Klebsiella pneumoniae, Sterptococcus pneumoniae compared to the Artificial honey.

Paper Citation

in Harvard Style

Bacayo M., Shafi S., Choon W., Fattepur S., Nilugal K., Khan J., Asmani F. and Yusuf E. (2018). A Comparative Study of the Antimicrobial Activity of Wild (Tualang) Honey and Artificial Honey Against Methecillin- resistant Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella Pneumoniae.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 111-117. DOI: 10.5220/0008358301110117

in Bibtex Style

@conference{bromo18,
author={May Florence Dela Cruz Bacayo and Shazwan Fahmi Shafi and Wong Charng Choon and Santosh Fattepur and Kiran Chanabasappa Nilugal and Jiyauddin Khan and Fadli Asmani and Eddy Yusuf},
title={A Comparative Study of the Antimicrobial Activity of Wild (Tualang) Honey and Artificial Honey Against Methecillin- resistant Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella Pneumoniae},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={111-117},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008358301110117},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - A Comparative Study of the Antimicrobial Activity of Wild (Tualang) Honey and Artificial Honey Against Methecillin- resistant Staphylococcus Aureus, Streptococcus Pneumoniae and Klebsiella Pneumoniae
SN - 978-989-758-347-6
AU - Bacayo M.
AU - Shafi S.
AU - Choon W.
AU - Fattepur S.
AU - Nilugal K.
AU - Khan J.
AU - Asmani F.
AU - Yusuf E.
PY - 2018
SP - 111
EP - 117
DO - 10.5220/0008358301110117