The Activity of Sterculia quadrifida R.br Stembark against Hepatitis C Virus

Maria Ayu Wandira Moi Sola, Adita Ayu Permanasari, Myrna Adianti, Lidya Tumewu, Aty Widyawaruyanti, Achmad Fuad Hafid

Abstract

Background: Sterculia quadrifida R.br is commonly known in East Nusa Tenggara, Indonesia as “Faloak”. Its stembark has been traditionally used to cure liver disease. One of the active agentscausing liver disease is the Hepatitis C Virus (HCV). Meanwhile, there is no information on the activity of this plant for anti-HCV. Objective: This study was aimed to investigate the anti-HCV activity and cytotoxicity of extracts and fractions from S. quadrifida R.br stembark. Methods: The stembark of S. quadrifida R.br was extracted using several different solvents. The stembark was gradually extracted using n-hexane, dichloromethane, and methanol. It was also extracted using 70% ethanol by ultrasonic-assisted extraction method as well. In addition, it was extracted using water by decocta method. All samples were further analyzed for their anti-HCV activity using Huh7it cell and HCV JFH1a virus, while the cytotoxicity was determined by MTT assay. The most active extract was further separated by column chromatography and the fractions were tested for their anti-HCV activity and cytotoxicity. Results: The anti-HCV assay results showed that water, 70% ethanol, and methanol were active against HCV with an IC 50 value of 6.06 µg/ml, 9.44 µg/ml, and 10.39 µg/ml, respectively. Meanwhile, the hexane and dichloromethane extracts were less active against HCV with IC 50 values of 51.93 µg/ml and 179.31 µg/ml, respectively. The fractionation of water extract as the most active extract resulted in seven fractions. Fractions 5 and 6 showed highest activity with IC 50 values of 7.60 µg/ml and 8.87 µg/ml, respectively. Furthermore, the cytotoxicity of these two active fractions exhibited no toxicity with CC 50 value of >2,000 µg/ml. Conclusion: Methanol extract, 70% ethanol extract, water extract, fraction 5, and fraction 6 of water extract from S. quadrifida R.br stem bark had potential activity as anti-HCV.

Paper Citation

in Harvard Style

Moi Sola M., Permanasari A., Adianti M., Tumewu L., Widyawaruyanti A. and Hafid A. (2018). The Activity of Sterculia quadrifida R.br Stembark against Hepatitis C Virus.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 106-110. DOI: 10.5220/0008358201060110

in Bibtex Style

@conference{bromo18,
author={Maria Ayu Wandira Moi Sola and Adita Ayu Permanasari and Myrna Adianti and Lidya Tumewu and Aty Widyawaruyanti and Achmad Fuad Hafid},
title={The Activity of Sterculia quadrifida R.br Stembark against Hepatitis C Virus},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={106-110},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008358201060110},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - The Activity of Sterculia quadrifida R.br Stembark against Hepatitis C Virus
SN - 978-989-758-347-6
AU - Moi Sola M.
AU - Permanasari A.
AU - Adianti M.
AU - Tumewu L.
AU - Widyawaruyanti A.
AU - Hafid A.
PY - 2018
SP - 106
EP - 110
DO - 10.5220/0008358201060110