In Vitro Study of DNA Adduct 8-hydroxy-2’ deoxyguanosine (8-OHdG) Formation Based on Fenton-like Reaction Using Chromium (III) and Benzo[a]pyrene

Budiawan, Intan Cahaya Dani, Sri Handayani, Filia Stephanie, Ridla Bakri

Abstract

In this research, study of 8-hydroxy-2’-deoxyguanosine (8-OHdG) caused by exposure of Chromium (III) and Benzo[a]pyrene was conducted. This study was done by reacting 2’-deoxyguanosine as DNA base with xenobiotic like Benzo[a]pyrene with variation of pH (7.4 and 8.4), incubation time (7 and 12 hours), and incubation temperature (37 o C and 60 o C). On this mixture, another observation was conducted with addition of Chromium (III) and H 2 O 2 as the Fenton-like reaction reagent. 8-OHdG DNA Adduct was then analyzed with High Performance Liquid Chromatography (HPLC) reversed phase with UV detector on 245 nm wavelength. The mixture of pH 6.7 Phospate Buffer 10mM and LC-grade methanol with ratio of 85:15 and 1 mL/minute flow rate were used in the measurement of 8-OHdG. On every mixture in all pH, time, and temperature variation, 8-OHdG was detected with the concentration below the Limit of Quantification, thus the concentration cannot be quantified. Addition of the Fenton like reaction reagent also impacted on higher 8-OHdG concentration in result. Longer incubation time and higher incubation temperature were proved to generate more 8-OHdG, meanwhile the variation of pH did not significantly affect the concentration of generated 8-OHdG in the mixture.

Paper Citation

in Harvard Style

Budiawan., Dani I., Handayani S., Stephanie F. and Bakri R. (2018). In Vitro Study of DNA Adduct 8-hydroxy-2’ deoxyguanosine (8-OHdG) Formation Based on Fenton-like Reaction Using Chromium (III) and Benzo[a]pyrene.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 88-93. DOI: 10.5220/0008357900880093

in Bibtex Style

@conference{bromo18,
author={Budiawan and Intan Cahaya Dani and Sri Handayani and Filia Stephanie and Ridla Bakri},
title={In Vitro Study of DNA Adduct 8-hydroxy-2’ deoxyguanosine (8-OHdG) Formation Based on Fenton-like Reaction Using Chromium (III) and Benzo[a]pyrene},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={88-93},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008357900880093},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - In Vitro Study of DNA Adduct 8-hydroxy-2’ deoxyguanosine (8-OHdG) Formation Based on Fenton-like Reaction Using Chromium (III) and Benzo[a]pyrene
SN - 978-989-758-347-6
AU - Budiawan.
AU - Dani I.
AU - Handayani S.
AU - Stephanie F.
AU - Bakri R.
PY - 2018
SP - 88
EP - 93
DO - 10.5220/0008357900880093