function (Katzung et al 2012) . Hence there is an
increase in demand to produce anti hypolipidemic
activity from the natural source.
According to the toxicity test result, MEIB extracts
produced non-toxic symptoms or mortality up to
dose level of 2000mg/kg body weight orally in rats
and hence the drug was considered safe for further
pharmacological screening. Phytochemical analysis
of the MEIB extracts revealed the presence of
alkaloid, flavonoid, terpenoid and tannins.
In the present study, the anti-cholesterol activity of
the test extract was measured by inducing
hypercholesterolemia in rats model. MEIB extracts
was used to evaluate the anti-cholesterol in this
hypercholesterolemia induced rats. The doses of the
test extract used for this study are 200mg/kg and
400mg/kg body weight respectively. The result
obtained from this study has been showed in lipid
profile of rats where 200mg/kg of MEIB extracts
shown to have non-significant anti-cholesterol effect
(P>0.05), whereas 400mg/kg of MEIB extracts has
significant anti-cholesterol effect (P<0.05) when
compared to the positive control. The results are
statistically analyzed using One Way ANOVA.
From previous studies, it showed that flavonoids
have the ability to reduce LDL-C and increase HDL-
C in hypercholesterolemia induced rats. The
antihyperlipidemic activity may also be attributed to
some of its active principles. The hypolipidemic
activity of natural products can be correlated to the
presence of flavonoids due to their properties of
inhibiting cholesterol biosynthesis and absorption
and modifying the activity of lipogenic and lipolytic
enzymes, leading to reduced lipid metabolism
(Borradaile et al 2003). So in this study, Flavonoids
presence in BPR extract might be the reason for
reducing TC, LDL-C and increasing HDL-C in
400mg/kg treated rats. Further experiments are
required to prove the exact mechanism and active
ingredients involved in the hypolipidemic activity.
5 CONCLUSION
The findings of the study revealed that MEIB extracts
are having anti-cholesterol properties with the dose
of 400mg/kg, and the anti-cholesterol effect is not
significant with 200mg/kg. This was proven
statistically with that P> 0.05.
Therefore, MEIB extracts could be a potential herbal
medicine as adjuvant with existing therapy for the
treatment of hyperlipidemia. Further studies to
isolate, identify, and characterize the active
principle(s) present in the extract have to be done.
ACKNOWLEDGEMENTS
The author is thankful to Professor Tan Sri Dato'
Wira Dr Mohd Shukri Ab Yajid, President,
Management & Science University, Malaysia, for
providing financial help and providing facilities to
complete the research.
CONFLICT OF INTERESTS
Authors shows no conflict of interests.
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