Enzymatic Esterification Ethyl Ester Fatty Acid from Hydrolyzed Castor Oil and Its Oxidation Product as Emulsifier and Antimicrobial Compound Using Candida rugosa Lipase E.C.3.1.1.3

Annisa Khairani, Sumi Hudiyono, Sri Handayani

Abstract

Aim of this study was to synthesis fatty acid ethyl ester compound of hydrolyzed castor oil and its oxidation product using Candida rugosa lipase. Both of the esterification products were expected to have antimicrobial activity against Staphylococcus epidermidis and Propionibacterium acnes and may act as emulsifiers. Optimization of esterification reactions was done by varying the mole ratio between fatty acids and ethanol, ie 1:1, 1:2, 1:3, and 1:4. The esterification products were then characterized using FTIR. Conversion percentage was determined by titrimetric method, to calculate the amount of fatty acids that was converted to ester. The ester product was tested its ability as emulsifier by emulsifier test. The esterification products were also examined their antimicrobial activity using disc diffusion method. The highest conversion percentage for fatty acid ethyl ester and its oxidation product were 76 % and 72% respectively. Characterization using FTIR for both ester showed that the absorption band of C=O ester functional group at wave number 1731.27 cm -1 and 1732.15 cm -1 respectively. The emulsifier test showed that both esters have ability to stabilize emulsion up to 24 hours for water-in-oil emulsion (w/o) type. Antimicrobial assay showed that both esters have antimicrobial activity against both bacteria.

Paper Citation

in Harvard Style

Khairani A., Hudiyono S. and Handayani S. (2018). Enzymatic Esterification Ethyl Ester Fatty Acid from Hydrolyzed Castor Oil and Its Oxidation Product as Emulsifier and Antimicrobial Compound Using Candida rugosa Lipase E.C.3.1.1.3.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 43-49. DOI: 10.5220/0008357200430049

in Bibtex Style

@conference{bromo18,
author={Annisa Khairani and Sumi Hudiyono and Sri Handayani},
title={Enzymatic Esterification Ethyl Ester Fatty Acid from Hydrolyzed Castor Oil and Its Oxidation Product as Emulsifier and Antimicrobial Compound Using Candida rugosa Lipase E.C.3.1.1.3},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={43-49},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008357200430049},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Enzymatic Esterification Ethyl Ester Fatty Acid from Hydrolyzed Castor Oil and Its Oxidation Product as Emulsifier and Antimicrobial Compound Using Candida rugosa Lipase E.C.3.1.1.3
SN - 978-989-758-347-6
AU - Khairani A.
AU - Hudiyono S.
AU - Handayani S.
PY - 2018
SP - 43
EP - 49
DO - 10.5220/0008357200430049