Study of Enzymatic Synthesis of Glycol – Castor Oil Fatty Acid and Glycol – Palmitic Acid Esters as Emulsifier and Antimicrobial Compounds Using Candida rugosa Lipase EC. 3.1.1.3

Adinda Azkia, Sumi Hudiyono, Sri Handayani

Abstract

This study was conducted to synthesize glycol – castor oil fatty acid and glycol – palmitic acid esters using Candida rugosa lipase as biocatalyst. The ester products was expected to have emulsifier and antimicrobial properties. Esterification was conducted by reacting fatty acid and glycol at 37 ⁰C for 18 hours. The variation of fatty acid mol ratio to glycol used were 1:1, 1:2, 1:3, and 1:4. The ester product was characterized using FTIR and the conversion percentage was determined by titrimetric method. Emulsifier test also performed to determine the ability of ester product as emulsifier. Antimicrobial assay were also conducted using disc diffusion method against Propionibacterium acne and Staphylococcus epidermidis. FTIR spectra for glycol – castor oil fatty acid and glycol – palmitic esters showed the absorption of C=O functional groups at wave numbers 1732.27 and 1741.88 cm -1 , respectively. The highest conversion percentage value for glycol – castor oil fatty acid and glycol – palmitic ester were 85% and 82%, respectively. The emulsifier test showed both glycol – fatty acid ester have properties as emulsifiers. Antimicrobial assay showed that glycol – castor oil fatty acid ester has activity as antimicrobial against both bacteria. However, glycol – palmitic ester has no activity as an antimicrobial agent.

Paper Citation

in Harvard Style

Azkia A., Hudiyono S. and Handayani S. (2018). Study of Enzymatic Synthesis of Glycol – Castor Oil Fatty Acid and Glycol – Palmitic Acid Esters as Emulsifier and Antimicrobial Compounds Using Candida rugosa Lipase EC. 3.1.1.3.In Proceedings of BROMO Conference - Volume 1: BROMO, ISBN 978-989-758-347-6, pages 5-11. DOI: 10.5220/0008356800050011

in Bibtex Style

@conference{bromo18,
author={Adinda Azkia and Sumi Hudiyono and Sri Handayani},
title={Study of Enzymatic Synthesis of Glycol – Castor Oil Fatty Acid and Glycol – Palmitic Acid Esters as Emulsifier and Antimicrobial Compounds Using Candida rugosa Lipase EC. 3.1.1.3},
booktitle={Proceedings of BROMO Conference - Volume 1: BROMO,},
year={2018},
pages={5-11},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008356800050011},
isbn={978-989-758-347-6},
}

in EndNote Style

TY - CONF

JO - Proceedings of BROMO Conference - Volume 1: BROMO,
TI - Study of Enzymatic Synthesis of Glycol – Castor Oil Fatty Acid and Glycol – Palmitic Acid Esters as Emulsifier and Antimicrobial Compounds Using Candida rugosa Lipase EC. 3.1.1.3
SN - 978-989-758-347-6
AU - Azkia A.
AU - Hudiyono S.
AU - Handayani S.
PY - 2018
SP - 5
EP - 11
DO - 10.5220/0008356800050011