Effect of Macrophage Addition and Incubation Time to Interferon Gamma (IFN-g) Levels on Tuberculosis Granulomas Invitro Models

Yusuf Eko Nugroho, Agung Dwi Wahyu Widodo, Jusak Nugraha

2018

Abstract

Tuberculosis (TB) is an infectious disease caused by stem bacteria Mycobacterium tuberculosis (Mtb) which can cause latent infection. Mtb enters through aerosolization which then infects and activates macrophages and dendritic cells present in the lungs. The active dendritic cells of coumadin present an antigen which has been processed in peptide form into the lymphocyte cell which later becomes granuloma. Granulomas are compounds of tissue composed of infected macrophages and multinucleated giant cells, known by aggregates of new monocytes or macrophages, and neutrophils and lymphocytes. Macrophages have a duty to kill Mtb germs. Different types of macrophage phenotype in granulomas with various functions, including anti-mycobacterial effector action, produce cytokines ie IFN-γ. In humans, IFN-g, released by activated Th1 cells, is the major lymphokine to activate the search process and antimicrobial activity that eliminates mycobacteria. This study aims to determine the relationship between IFN-g and In-vitro method using PBMC infected with Mtb germ with the addition of Macrophage with concentration 1,2,3x105 with incubation time 1,2,3,4 and 5 days to determine the level of IFN -g using ELISA test. With use with day variables of macrophages 1x105, 2x105, and 3x105 analized with One-Way ANOVA obtained values ​​p = P = 0.7201 The value is greater than 0.05 (p> 0.05). There is no meaningful difference.

Paper Citation

in Harvard Style

Nugroho Y., Widodo A. and Nugraha J. (2018). Effect of Macrophage Addition and Incubation Time to Interferon Gamma (IFN-g) Levels on Tuberculosis Granulomas Invitro Models.In Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS, ISBN 978-989-758-348-3, pages 392-397. DOI: 10.5220/0007543603920397

in Bibtex Style

@conference{icps18,
author={Yusuf Eko Nugroho and Agung Dwi Wahyu Widodo and Jusak Nugraha},
title={Effect of Macrophage Addition and Incubation Time to Interferon Gamma (IFN-g) Levels on Tuberculosis Granulomas Invitro Models},
booktitle={Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,},
year={2018},
pages={392-397},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007543603920397},
isbn={978-989-758-348-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd International Conference Postgraduate School - Volume 1: ICPS,
TI - Effect of Macrophage Addition and Incubation Time to Interferon Gamma (IFN-g) Levels on Tuberculosis Granulomas Invitro Models
SN - 978-989-758-348-3
AU - Nugroho Y.
AU - Widodo A.
AU - Nugraha J.
PY - 2018
SP - 392
EP - 397
DO - 10.5220/0007543603920397