Distribution and Phylogenetic Diversity of CbbL Gene Encoding RuBisCo in the Deep-sea Sediments from the South China Sea

Jie Su, Hongxia Ming, Quanrui Chen, Yuan Jin, Chunxin Zhang, Daoming Guan, Jingfeng Fan

2018

Abstract

RuBisCO is the key enzyme catalyzing the first and major step of carbon fixation in Calvin cycle. With the aim to examine the phylogenetic diversity of RuBisCO genes, cbbL gene was amplified by PCR from three South China Sea deep-sea sample, cloned, and sequenced. A total of 28 OTUs deriving from 236 cbbL clones covered 16 phylotypes which all belong to Proteobacteria. The estimated coverage values showed that more than 85% of bacterial cbbL diversity was captured. Shannon and Simpson indices indicated a high diversity of the total cbbL gene in the SCS deep-sea sediments. In conclusion, microbial RuBisCO genes in the South China Sea display a broad range of phylogenetic diversity. The predominant group in these three deep-sea sediments included Thiobacillus and Thiorhodococcus, which was chemoautotrophic bacteria involving in Calvin–Benson–Bassham cycle. The above results implicate that bacteria with the potential for carbon dioxide fixation and chemoautotrophy oocur in the South China Sea.

Paper Citation

in Harvard Style

Su J., Ming H., Chen Q., Jin Y., Zhang C., Guan D. and Fan J. (2018). Distribution and Phylogenetic Diversity of CbbL Gene Encoding RuBisCo in the Deep-sea Sediments from the South China Sea.In Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG, ISBN 978-989-758-342-1, pages 5-10. DOI: 10.5220/0007424900050010

in Bibtex Style

@conference{iweg18,
author={Jie Su and Hongxia Ming and Quanrui Chen and Yuan Jin and Chunxin Zhang and Daoming Guan and Jingfeng Fan},
title={Distribution and Phylogenetic Diversity of CbbL Gene Encoding RuBisCo in the Deep-sea Sediments from the South China Sea},
booktitle={Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG,},
year={2018},
pages={5-10},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007424900050010},
isbn={978-989-758-342-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Workshop on Environment and Geoscience - Volume 1: IWEG,
TI - Distribution and Phylogenetic Diversity of CbbL Gene Encoding RuBisCo in the Deep-sea Sediments from the South China Sea
SN - 978-989-758-342-1
AU - Su J.
AU - Ming H.
AU - Chen Q.
AU - Jin Y.
AU - Zhang C.
AU - Guan D.
AU - Fan J.
PY - 2018
SP - 5
EP - 10
DO - 10.5220/0007424900050010