AUTOMATED DETECTION OF INTERPHASE AND METAPHASE NUCLEI IN THE FISH IMAGES

Jan Schier, Bohumil Kovár, Eduard Kocárek

2012

Abstract

The fluorescence in-situ hybridization (FISH) belongs to the most common cytogenetic methods and is widely applied in routine clinical genetic diagnostics. We are paying attention to FISH analysis of chromosomal aneuploidies – the deviations from chromosomal number. Such analysis is based on evaluation of up to several hundreds of microscopic images. Computer support for this process includes using methods of image processing and data mining. In this paper, we focus on the image processing part in more detail: first, the properties of FISH images are reviewed, then, the processing flow is outlined. Our aim is to find the interphase and metaphase nuclei and the hybridization signals contained in the image. A simple method using the raw and central moments of detected objects as measures to distinguish between the two types of nuclei is proposed.

References

  1. Annerén, G. (2007). Turner syndrome. The American Journal of Human Genetics, 68(3):2-9. John Wiley & Sons, Ltd.
  2. Beatty, B., Mai, S., and Squire, J. (2002). FISH. Oxford University Press, Oxford.
  3. Harper, P. S. (2004). Practical genetic counselling. Arnold, London, 6 edition.
  4. Otsu, N. (1979). A threshold selection method from graylevel histograms. IEEE Transactions on Systems, Man, and Cybernetics, 9:62-66.
  5. Rittscher, J., Machiraju, R., and Wong, S. T. C., editors (2008). Microscopic Image Analysis for Life Science Applications. Artech House, Inc.
  6. Roizen, N. and Patterson, D. (2003). Down's syndrome. The Lancet, 361(9365):1281-1289.
  7. Varella-Garcia, M. (2003). Molecular cytogenetics in solid tumors: Laboratorial tool for diagnosis, prognosis, and therapy. The Oncologist, 8(3):45-48.
  8. Visootsak, J. and Graham, J. (2006). Klinefelter syndrome and other sex chromosomal aneuploidies. Orphanet Journal of Rare Diseases, 1(42). Biomed Central.
Download


Paper Citation


in Harvard Style

Schier J., Kovár B. and Kocárek E. (2012). AUTOMATED DETECTION OF INTERPHASE AND METAPHASE NUCLEI IN THE FISH IMAGES . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2012) ISBN 978-989-8425-90-4, pages 347-350. DOI: 10.5220/0003797703470350


in Bibtex Style

@conference{bioinformatics12,
author={Jan Schier and Bohumil Kovár and Eduard Kocárek},
title={AUTOMATED DETECTION OF INTERPHASE AND METAPHASE NUCLEI IN THE FISH IMAGES},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2012)},
year={2012},
pages={347-350},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003797703470350},
isbn={978-989-8425-90-4},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2012)
TI - AUTOMATED DETECTION OF INTERPHASE AND METAPHASE NUCLEI IN THE FISH IMAGES
SN - 978-989-8425-90-4
AU - Schier J.
AU - Kovár B.
AU - Kocárek E.
PY - 2012
SP - 347
EP - 350
DO - 10.5220/0003797703470350