Authors:
Dian Yuliartha Lestari
and
Faiq Shabri Maulana
Affiliation:
University of Muhammadiyah Malang, Indonesia
Keyword(s):
Malang Apple Cider, Mast Cell, Ovalbumin, Allergic Reaction
Abstract:
Allergy is a state of hypersensitivity induced by exposure to certain antigens that cause reactions immunology. Mast cells have a role in triggering allergies. There is a new innovation as an anti-allergy, one of them is apple because it contains quercetin. Quercetin are believed to have an influential effect on human health, that of anti-allergy and anti-inflammatory activity. This study to determine the effectiveness of Malang Apple Cider (Malus sylvestris) on decreasing the number of mast cells in the lung of an allergic induced rat (Rattus novergicus). This study was experimental research using Post Test Control Group Design. Samples were white male Wistar Strain Rats which were divided into 5 groups, each group contains 5 rats. The positive control group was induced with ovalbumin through intraperitoneal and inhalation. Besides ovalbumin induction, treatment groups were also given apple cider with doses of 15%, 20%, and 25%. Lung tissue was taken and then paraffin blocks were cr
eated and stained using Methylene blue staining and evaluated with the microscope in 40 x magnification. Data analyzed using One Way ANOVA. Statistical results showed there was no significant (p = 0,079 > or p 0.05). This decline is due to the levels of Quercetin in apple cider can prevent mast cell degranulation. However, the decrease was not significant occurs due to several factors, including the strength of apple cider, the absorption in the intestine, stress factors, and environmental circumstances.
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