Photoinactivation of Methicillin-Resistant S. Aureus Biofilm using a New Chlorin as Photosensitizer

L. S. Amaral, I. A. P. Linares, J. R. Perussi, J. R. Perussi

2020

Abstract

Due to the increase in bacterial resistance to antibiotics, the development of new drugs and technologies for the eradication of microorganisms is a priority. Photodynamic Therapy (PDT) depends on the interaction between a light-sensitive compound (photosensitizer), light, and molecular oxygen. The reaction generates reactive oxygen species (ROS), which induce cell death by oxidative stress. Antimicrobial Photodynamic Therapy (A-PDT) may be a promising alternative for microbial infections since its action occurs by multiple targets, which hinders the development of resistance. The main goal of this study was the evaluation of the potential of a newly synthesized chlorin derivative sterically prevented from self-aggregation as a photosensitizer to photoinactivation Methicillin-Resistant S. aureus (MRSA) biofilm and to investigate the membrane integrity after the treatment. The results showed a high potential of this chlorin for photoinactivation of MRSA biofilms reducing the survival index more than 5 log CFU mL-1 leading to the unstructured membrane and consequent cell death by photooxidation of membrane components after A-PDT.

Paper Citation

in Harvard Style

Amaral L., Linares I. and Perussi J. (2020). Photoinactivation of Methicillin-Resistant S. Aureus Biofilm using a New Chlorin as Photosensitizer. In Proceedings of the 8th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS, ISBN 978-989-758-401-5, pages 92-96. DOI: 10.5220/0008938100920096

in Bibtex Style

@conference{photoptics20,
author={L. Amaral and I. Linares and J. Perussi},
title={Photoinactivation of Methicillin-Resistant S. Aureus Biofilm using a New Chlorin as Photosensitizer},
booktitle={Proceedings of the 8th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,},
year={2020},
pages={92-96},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008938100920096},
isbn={978-989-758-401-5},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 8th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,
TI - Photoinactivation of Methicillin-Resistant S. Aureus Biofilm using a New Chlorin as Photosensitizer
SN - 978-989-758-401-5
AU - Amaral L.
AU - Linares I.
AU - Perussi J.
PY - 2020
SP - 92
EP - 96
DO - 10.5220/0008938100920096