Extraction Optimization of Total Phenolic Content and Antioxidant Activity from Teaw (Cratoxylum formosum) by Central Composite Design

Nuttakarn Somarrom, Varipat Areekul

2019

Abstract

The extraction of bioactive compounds from Teaw (Cratoxylum formosum (Jack) Dyer) was carried out based on the central composite design and response surface methodology to optimize the three variables: solvent polarity index (5.5-9.0), temperature (50-70°C) and extraction time (35-55 min). The optimization condition was based on yield, total phenolic content (TPC), DPPH radical scavenging (DPPH), ABTS radical scavenging (ABTS) and thiobarbituric acid reactive substances (TBARs) assay. From five variables, only two responses (yield and TPC) were fitted to a quadratic equation with R-square of 0.8479 and 0.8891, respectively. DPPH response was not quite fitted (R2 = 0.4420) while ABTS and TBARs were insignificantly fitted (p>0.05). The optimal extraction condition was obtained with a solvent polarity index of 8.64 at 70°C for 55 min. The software predicted under these optimal conditions that yield was 28.58% whereas the TPC and DPPH, were 289 and 402 mg/g dry sample, respectively. The desirability value was 0.933. The proposed method proves to be simple, cheap and good for natural bioactive extraction from Teaw, being a potential approach for natural antioxidants.

Paper Citation

in Harvard Style

Somarrom N. and Areekul V. (2019). Extraction Optimization of Total Phenolic Content and Antioxidant Activity from Teaw (Cratoxylum formosum) by Central Composite Design. In Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC, ISBN 978-989-758-467-1, pages 119-125. DOI: 10.5220/0009980700002964

in Bibtex Style

@conference{16th afc19,
author={Nuttakarn Somarrom and Varipat Areekul},
title={Extraction Optimization of Total Phenolic Content and Antioxidant Activity from Teaw (Cratoxylum formosum) by Central Composite Design},
booktitle={Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,},
year={2019},
pages={119-125},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0009980700002964},
isbn={978-989-758-467-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 16th ASEAN Food Conference - Volume 1: 16th AFC,
TI - Extraction Optimization of Total Phenolic Content and Antioxidant Activity from Teaw (Cratoxylum formosum) by Central Composite Design
SN - 978-989-758-467-1
AU - Somarrom N.
AU - Areekul V.
PY - 2019
SP - 119
EP - 125
DO - 10.5220/0009980700002964