Dengue Virus (DENV)-1 Induces High Expression of Anti- and Pro-inflammatory Cytokines in Human Macrophages

Winda Yulia, Benediktus Yohan, Febrina Meutiawati, Alida R. Harahap, R. Tedjo Sasmono

2018

Abstract

Dengue is caused by dengue virus (DENV) infection. The pathological mechanism of dengue infection is still poorly understood. Serotype variation and host’s immune response are thought to have roles in disease severity. This study compared the ability of four DENV serotypes (DENV-1, -2,-3,-4) isolates from Indonesia to induce the expression of anti- and pro-inflammatory cytokines in Monocyte-Derived Macrophages (MDMs) differentiated from a healthy human. Monocytes were isolated from healthy human donors and differentiated into macrophages under the stimulation of Macrophage Colony Stimulating Factor (M-CSF). Mature MDMs were infected with four different serotypes of DENV isolates from Indonesia. The resulting expression kinetics of Interferon alpha (IFN-a2) and Interleukin-10 (IL-10) as anti-inflammatory cytokines and IL-1b as pro-inflammatory cytokine was measured at six different time points using Luminex immunoassay. The presence of DENV Non-Structural Protein 1 (NS1) as a marker of virus replication was also measured using ELISA. DENV-1 showed a tendency to induce the expression of IFNa2 and IL-10 more rapidly and at a higher level than other serotypes albeit of lower NS1 expression. The expression of IL-1b was down-regulated in response to all DENV serotypes infection. This study demonstrates the differences in the ability of four DENV serotypes in regulating the expression of cytokines in MDMs with DENV-1 showed a superior inducing capability compared to other serotypes. This data provides information of possible serotype-specific role on disease severity.

Paper Citation

in Harvard Style

Yulia W., Yohan B., Meutiawati F., Harahap A. and Sasmono R. (2018). Dengue Virus (DENV)-1 Induces High Expression of Anti- and Pro-inflammatory Cytokines in Human Macrophages.In Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS, ISBN 978-989-758-438-1, pages 44-48. DOI: 10.5220/0008790500440048

in Bibtex Style

@conference{skic-mhs18,
author={Winda Yulia and Benediktus Yohan and Febrina Meutiawati and Alida R. Harahap and R. Tedjo Sasmono},
title={Dengue Virus (DENV)-1 Induces High Expression of Anti- and Pro-inflammatory Cytokines in Human Macrophages},
booktitle={Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,},
year={2018},
pages={44-48},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0008790500440048},
isbn={978-989-758-438-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 2nd Syiah Kuala International Conference on Medicine and Health Sciences - Volume 1: SKIC-MHS,
TI - Dengue Virus (DENV)-1 Induces High Expression of Anti- and Pro-inflammatory Cytokines in Human Macrophages
SN - 978-989-758-438-1
AU - Yulia W.
AU - Yohan B.
AU - Meutiawati F.
AU - Harahap A.
AU - Sasmono R.
PY - 2018
SP - 44
EP - 48
DO - 10.5220/0008790500440048