The Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods on Down Regulation Cyclin D1 Expression against HeLa Cell Line

Aminah Dalimunthe, Poppy Anjelisa Zaitun Hasibuan, Denny Satria

2018

Abstract

Objective: This study was to determined the activity of Litsea cubeba Lour. heartwood alkaloid fraction on down regulation cyclin D1 expression towards HeLa cell line. Methods: The heartwood powder of Litsea cubeba Lour. was macerated by ethanol 96% and fractionated with n-hexane and chloroform at pH 3,7 and 9. Antiproliferation study was using MTT assay and cyclin D1 expression analysis was using flow cytometry method from chloroform fraction at pH 7. Results: The IC50 of chloroform fractions at pH 7 was 52.46 ± 0.34 µg/mL. The fractions of chloroform at pH 7 in concentration of 25 and 10 µg/mL were as antiproliferation assay with viable cells at 53.57 ± 0.28% and 68.43 ± 0.48% respectively after 72 h incubation and decreased cyclin D1 expression (69.10 and 67.63%). Conclusion: The results reveal that Litsea cubeba Lour. heartwood alkaloid fractions provide effective as anticancer with mechanism through down regulation of cyclin D1. Our further study is to isolate alkaloid compounds and assess its moleculer mechanism.

Paper Citation

in Harvard Style

Dalimunthe A., Hasibuan P. and Satria D. (2018). The Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods on Down Regulation Cyclin D1 Expression against HeLa Cell Line.In Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR, ISBN 978-989-758-449-7, pages 790-793. DOI: 10.5220/0010091107900793

in Bibtex Style

@conference{icosteerr18,
author={Aminah Dalimunthe and Poppy Anjelisa Zaitun Hasibuan and Denny Satria},
title={The Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods on Down Regulation Cyclin D1 Expression against HeLa Cell Line},
booktitle={Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,},
year={2018},
pages={790-793},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010091107900793},
isbn={978-989-758-449-7},
}

in EndNote Style

TY - CONF

JO - Proceedings of the International Conference of Science, Technology, Engineering, Environmental and Ramification Researches - Volume 1: ICOSTEERR,
TI - The Activity of Alkaloid Fraction of Litsea cubeba Lour. Heartwoods on Down Regulation Cyclin D1 Expression against HeLa Cell Line
SN - 978-989-758-449-7
AU - Dalimunthe A.
AU - Hasibuan P.
AU - Satria D.
PY - 2018
SP - 790
EP - 793
DO - 10.5220/0010091107900793