The Effect of Circadian Rhythm on Hematopoietic Stem Cell Mobilization in Peripheral Blood as a Result of Submaximal Physical Exercise

Dhoni Akbar Ghozali, Harjanto, Agung Dwi Wahyu Widodo

2017

Abstract

Abstract: Physical exercise triggers increased production of reactive oxygen species (ROS) causing skeletal muscle damage. Exercise also triggers an increase of granulocyte-colony stimulating factor (G-CSF), tumor necrosis factor (TNFα) and interleukin-6 (IL-6) as an inflammatory response of skeletal muscle damage playing a role in the mobilization of hematopoietic stem cells (HSC). Therefore, we should be aware of circadian rhythm during physical exercise in order to have positive health benefits for the body. It is known that ROS after submaximal physical exercise in an afternoon session was higher than it was in the morning. The aim of this study is to demonstrate HSC mobilization in peripheral blood as a result of submaximal exercise in a morning and afternoon session. HSC are the stem cells that express CD34+ in peripheral blood. HSC mobilization was observed from BD stem cell enumeration kit, Catalog No. 344563. Specimens were taken from venous blood before and 30 minutes after exercise, followed with peripheral blood mononuclear cell (PBMC) isolation process and flow cytometry. Using a modified Harvard step test, the morning submaximal physical exercise was performed at 08.00 to 09.00 while the afternoon submaximal physical exercise was performed at 15.00 to 16.00. The results show an increase in the percentage of HSC (%CD34+) after submaximal physical exercise in the morning and a decrease (%CD34+) after the afternoon exercise. Submaximal physical exercise in the morning was proven to increase HSC mobilization, and submaximal physical exercise in the afternoon was proven to decrease HSC mobilization.

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Paper Citation


in Harvard Style

Akbar Ghozali D., Harjanto. and Dwi Wahyu Widodo A. (2017). The Effect of Circadian Rhythm on Hematopoietic Stem Cell Mobilization in Peripheral Blood as a Result of Submaximal Physical Exercise.In Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS, ISBN 978-989-758-340-7, pages 48-52. DOI: 10.5220/0007332500480052


in Bibtex Style

@conference{sips17,
author={Dhoni Akbar Ghozali and Harjanto and Agung Dwi Wahyu Widodo},
title={The Effect of Circadian Rhythm on Hematopoietic Stem Cell Mobilization in Peripheral Blood as a Result of Submaximal Physical Exercise},
booktitle={Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS,},
year={2017},
pages={48-52},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0007332500480052},
isbn={978-989-758-340-7},
}


in EndNote Style

TY - CONF

JO - Proceedings of Surabaya International Physiology Seminar - Volume 1: SIPS,
TI - The Effect of Circadian Rhythm on Hematopoietic Stem Cell Mobilization in Peripheral Blood as a Result of Submaximal Physical Exercise
SN - 978-989-758-340-7
AU - Akbar Ghozali D.
AU - Harjanto.
AU - Dwi Wahyu Widodo A.
PY - 2017
SP - 48
EP - 52
DO - 10.5220/0007332500480052