THE CONSEQUENCES OF LOW FREQUENCY AND INTENSITY ELECTROMAGNETIC FIELDS ON THE FREQUENCY OF MICRONUCLEI IN HeLa CELLS

Cosmin Teodor Mihai, Gabriela Căpraru, Elena Truță, Pincu Rotinberg, Daniela Gherghel

Abstract

The treatment of HeLa neoplastic cells with low frequency and intensity electromagnetic field has determined modifications of the micronuclei number, this impact being correlated with the application manner of the electromagnetic field (continuous or discontinuous). Thus, the continuous electromagnetic field has reduced the frequency of the micronuclei formation (2.91 ± 0.015 ‰), as compared to the value of control group (3.93 ± 0.023 ‰), while the discontinuously applied electromagnetic field has increased the number of micronuclei (4.92± 0.012 ‰). These variations in micronuclei number suggested that low frequency electromagnetic field interfere in different ways with the genetic material of cancerous cells, indicating that the cEMF had a protective effect upon DNA molecule, while dcEMF had a genotoxic impact. Also, the estimation of the micronuclei area has revealed that the area of micronuclei generated by dcEMF was smaller than that of cEMF.

References

  1. Davies, E., Olliff, C., Wright, I., Woodward, A. & Kell, D. (1999). A weak pulsed magnetic field affects adenine nucleotide oscillations, and related parameters in aggregating Dictyostelium discoideum amoebae. Bioelectrochem Bioenerg, 48, 149-162.
  2. Falone, S., Grossi, M. R., Cinque, B., D'Angelo, B., Tettamanti, E., Cimini, A., Di Ilio, C. & Amicarelli, F. (2007). Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells. Int. J. Biochem. Cell Biol., 39, 2093-2106.
  3. Girgert, R., Gründker, C., Emons, G. & Hanf, V. (2008). Electromagnetic fields alter the expression of estrogen receptor cofactors in breast cancer cells. Bioelectromagnetics, 29, 169-176.
  4. Hardell, L. & Sage, C. (2008). Biological effects from electromagnetic field exposure and public exposure standards. Biomed. Pharmacother., 62, 104-109.
  5. Hee Cho, Y. & Chung Won, H. (2003). The effect of extremely low frequency electromagnetic fields (ELFEMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene. Toxicol. Lett., 143, 37-44.
  6. Heynick, L. N., Johnston, S. A. & Mason, P.A. (2003). Radio frequency electromagnetic fields: cancer, mutagenesis, and genotoxicity. Bioelectromagnetics, Suppl 6, S74-100.
  7. Johansson, O. (2009). Disturbance of the immune system by electromagnetic fields-A potentially underlying cause for cellular damage and tissue repair reduction which could lead to disease and impairment. Pathophysiology, 16, 157-177.
  8. Juutilainen, J. & Lang, S. (1997). Genotoxic, carcinogenic and teratogenic effects of electromagnetic fields. Introduction and overview. Mutat. Res., 387, 165-171.
  9. Juutilainen, J., Heikkinen, P., Soikkeli, H. & MäkiPaakkanen, J. (2007).Micronucleus frequency in erythrocytes of mice after long-term exposure to radiofrequency radiation. Int. J. Radiat. Biol., 83, 213- 220.
  10. Juutilainen, J., Kumlin, T. & Naarala, J. (2006). Do extremely low frequency magnetic fields enhance the effects of environmental carcinogens? A meta-analysis of experimental studies. Int. J. Radiat. Biol., 82, 1-12.
  11. Kavet, R. (1996). EMF and current cancer concepts. Bioelectromagnetics, 17, 339-357.
  12. Mairs, R. J., Hughes, K., Fitzsimmons, S., Prise, K. M., Livingstone, A., Wilson, L., Baig, N., Clark, A. M., Timpson, A., Patel, G., Folkard, M., Angerson, W. J. & Boyd, M. (2007). Microsatellite analysis for determination of the mutagenicity of extremely lowfrequency electromagnetic fields and ionising radiation in vitro. Mutat. Res., 626, 34-41.
  13. Meltz, M. L. (2003). Radiofrequency exposure and mammalian cell toxicity, genotoxicity, and transformation. Bioelectromagnetics, Suppl 6, S196- 213.
  14. Pasquini, R., Villarini, M., Sforzolini Scassellati, G., Fatigoni, C. & Moretti, M. (2003). Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol. Toxicology in Vitro, 17, 581-586.
  15. Ronchetto, F., Barone, D., Cintorino, M., Berardelli, M., Lissolo, S., Orlassino, R., Ossola, P. & Tofani, S. (2004). Extremely low frequency-modulated static magnetic fields to treat cancer: A pilot study on patients with advanced neoplasm to assess safety and acute toxicity. Bioelectromagnetics, 25, 563-571.
  16. Savage, J. R. K. (2000). Micronuclei : Pitfalls and Problems in Atlas of Genetics and Cytogenetics in Oncology and Haematology. Retrived July 2000 from http://atlasgeneticsoncology.org/Deep/MicronucleiID2 0016.html.
  17. Simkó, M., Kriehuber, R., Weiss, D. G. & Luben, R.A. (1998). Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines. Bioelectromagnetics, 19, 85-91.
  18. Speit, G., Schütz, P. & Hoffmann, H. (2007). Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in cultured mammalian cells are not independently reproducible. Mutat. Res., 626, 42-47.
  19. Tenuzzo, B., Chionna, A., Panzarini, E., Lanubile, R., Tarantino, P., Di Jeso, B., Dwikat, M. & Dini, L. (2006). Biological effects of 6 mT statistic magnetic fields: a comparative study in different cell types. Bioelectromagnetics, 27, 560-577.
  20. Thun-Battersby, S., Mevissen, M. & Löscher, W. (1999). Exposure of Sprague-Dawley rats to a 50-Hertz, 100- microTesla magnetic field for 27 weeks facilitates mammary tumorigenesis in the 7,12-dimethylbenz[a]- anthracene model of breast cancer. Cancer Res., 59, 3627-3633.
  21. Verschaeve, L., Heikkinen, P., Verheyen, G., Van Gorp, U., Boonen, F., Vander Plaetse, F., Maes, A., Kumlin, T., Mäki-Paakkanen, J., Puranen, L. & Juutilainen, J. (2006). Investigation of co-genotoxic effects of radiofrequency electromagnetic fields in vivo. Radiat. Res., 165, 598-607.
  22. Wolff, I. & Muller, P. (2005). Micronuclei and Comet Assay. In J. E. Celis, T. Hunter, N. Carter, D. Shotton, J. V. Small & K. Simons (Eds.), Cell Biology: A Laboratory Handbook. Elsevier Academic Press. pp. 325-334.
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in Harvard Style

Mihai C., Căpraru G., Truță E., Rotinberg P. and Gherghel D. (2011). THE CONSEQUENCES OF LOW FREQUENCY AND INTENSITY ELECTROMAGNETIC FIELDS ON THE FREQUENCY OF MICRONUCLEI IN HeLa CELLS . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 440-443. DOI: 10.5220/0003292804400443


in Bibtex Style

@conference{biodevices11,
author={Cosmin Teodor Mihai and Gabriela Căpraru and Elena Truță and Pincu Rotinberg and Daniela Gherghel},
title={THE CONSEQUENCES OF LOW FREQUENCY AND INTENSITY ELECTROMAGNETIC FIELDS ON THE FREQUENCY OF MICRONUCLEI IN HeLa CELLS},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={440-443},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003292804400443},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - THE CONSEQUENCES OF LOW FREQUENCY AND INTENSITY ELECTROMAGNETIC FIELDS ON THE FREQUENCY OF MICRONUCLEI IN HeLa CELLS
SN - 978-989-8425-37-9
AU - Mihai C.
AU - Căpraru G.
AU - Truță E.
AU - Rotinberg P.
AU - Gherghel D.
PY - 2011
SP - 440
EP - 443
DO - 10.5220/0003292804400443