A PDMS BASED INTEGRATED PCR MICROCHIP FOR GENETIC ANALYSIS

Sandeep Kumar Jha, You-Cheol Jang, Rohit Chand, Kamrul Islam, Yong-Sang Kim

Abstract

An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker (SMAD4) from non-tumorigenic MCF10a human cell lines. The 193 bp PCR amplicon obtained through on-chip PCR was confirmed in case of MCF 10a cells through agarose gel electrophoresis, whereas no product was detected in case of tumorigenic MCF7 cells. The total time required for entire reaction was less than 90 min. Therefore, we propose that such microchip can be helpful in predicting the risk of cancer by analysis of genetic tumor markers from human samples and can also be used for other genetic analysis involving PCR reaction.

References

  1. Dixit, V. and R. L. Juliano, 2008. Selective killing of SMAD4-negative tumor cells via a designed repressor strategy. Mol Pharmacol. 74(1) 289-97.
  2. Joung, S-R., Kang, C-J. and Kim Y-S, 2008. Series DNA Amplification Using the Continuous-flow Polymerase Chain Reaction Chip. Japanese Journal of Applied Physics. 47(2) 1342-1345.
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Paper Citation


in Harvard Style

Kumar Jha S., Jang Y., Chand R., Islam K. and Kim Y. (2011). A PDMS BASED INTEGRATED PCR MICROCHIP FOR GENETIC ANALYSIS . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011) ISBN 978-989-8425-37-9, pages 273-275. DOI: 10.5220/0003288202730275


in Bibtex Style

@conference{biodevices11,
author={Sandeep Kumar Jha and You-Cheol Jang and Rohit Chand and Kamrul Islam and Yong-Sang Kim},
title={A PDMS BASED INTEGRATED PCR MICROCHIP FOR GENETIC ANALYSIS},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)},
year={2011},
pages={273-275},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003288202730275},
isbn={978-989-8425-37-9},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2011)
TI - A PDMS BASED INTEGRATED PCR MICROCHIP FOR GENETIC ANALYSIS
SN - 978-989-8425-37-9
AU - Kumar Jha S.
AU - Jang Y.
AU - Chand R.
AU - Islam K.
AU - Kim Y.
PY - 2011
SP - 273
EP - 275
DO - 10.5220/0003288202730275