TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS

Stefan Sokoll, Klaus Tönnies, Martin Heine

Abstract

Studying molecular dynamics is crucial for understanding biological processes in living cells. In principle, this is achieved by attaching fluorescent particles to molecules of interest and their detection using fluorescence microscopy. These analysis require fast optical techniques with at least 20Hz frame rate and a resolution below the diffraction limit in all three spatial dimensions. Current approaches basically rely on determining the correlation between features of the particle’s 2D point spread function (PSF) and the focal distance to the center of the particle. However, they are still unsuitable for the application to live cell imaging where the refractive index mismatch is present. This mismatch leads to non-stationary optical properties of the particles on which the algorithms rely, necessitating a calibration procedure prior to every experiment. However, this is almost unfeasible to particles attached to living cells. We established a spinning disk confocal setup and employ Quantum dots (QD) as fluorescence particles. Corresponding models of the axial PSF features that define the distance to the center of the particle are developed and analyzed in the presence of the refractive index mismatch. We present this analysis as the base for the future development of a 3D localization technique applicable to living cells.

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Paper Citation


in Harvard Style

Sokoll S., Tönnies K. and Heine M. (2011). TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS . In Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011) ISBN 978-989-8425-36-2, pages 210-215. DOI: 10.5220/0003166502100215


in Bibtex Style

@conference{bioinformatics11,
author={Stefan Sokoll and Klaus Tönnies and Martin Heine},
title={TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS},
booktitle={Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)},
year={2011},
pages={210-215},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003166502100215},
isbn={978-989-8425-36-2},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms - Volume 1: BIOINFORMATICS, (BIOSTEC 2011)
TI - TOWARDS FAST 3D NANOPARTICLE LOCALIZATION FOR STUDYING MOLECULAR DYNAMICS IN LIVING CELLS
SN - 978-989-8425-36-2
AU - Sokoll S.
AU - Tönnies K.
AU - Heine M.
PY - 2011
SP - 210
EP - 215
DO - 10.5220/0003166502100215