ON-CHIP FLUORESCENCE LIFETIME EXTRACTION USING SYNCHRONOUS GATING SCHEME - Theoretical Error Analysis and Practical Implementation

Day-Uei Li, Bruce Rae, David Renshaw, Robert Henderson, Eleanor Bonnist

2008

Abstract

A synchronous gating technique was proposed for fluorescent photon collecting. The two- and multi-gate rapid lifetime determination (RLD) technique was applied to implement on-chip fluorescence lifetime extraction. Compared with all available iterative least square method (LSM) or maximum likelihood estimation (MLE) based general purpose FLIM analysis software, we offer a method for the direct calculation of lifetime based on the photon counts stored in on-chip memory and deliver faster analysis to enable real-time applications. Theoretical error analysis of the two-gate RLD technique was derived for comparison. The performance of the algorithms were tested on a single-exponential histogram obtained from a CMOS SPAD detector chip using a 468nm laser diode light source with optimized gate width. Moreover, a multi-exponential pipelined RLD FLIM technique was also proposed and tested on a four- exponential decay DNA sample containing a single adenine analogue 2-aminopurine.

Paper Citation

in Harvard Style

Li D., Rae B., Renshaw D., Henderson R. and Bonnist E. (2008). ON-CHIP FLUORESCENCE LIFETIME EXTRACTION USING SYNCHRONOUS GATING SCHEME - Theoretical Error Analysis and Practical Implementation . In Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008) ISBN 978-989-8111-18-0, pages 171-176. DOI: 10.5220/0001061301710176

in Bibtex Style

@conference{biosignals08,
author={Day-Uei Li and Bruce Rae and David Renshaw and Robert Henderson and Eleanor Bonnist},
title={ON-CHIP FLUORESCENCE LIFETIME EXTRACTION USING SYNCHRONOUS GATING SCHEME - Theoretical Error Analysis and Practical Implementation},
booktitle={Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008)},
year={2008},
pages={171-176},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001061301710176},
isbn={978-989-8111-18-0},
}

in EndNote Style

TY - CONF
JO - Proceedings of the First International Conference on Bio-inspired Systems and Signal Processing - Volume 2: BIOSIGNALS, (BIOSTEC 2008)
TI - ON-CHIP FLUORESCENCE LIFETIME EXTRACTION USING SYNCHRONOUS GATING SCHEME - Theoretical Error Analysis and Practical Implementation
SN - 978-989-8111-18-0
AU - Li D.
AU - Rae B.
AU - Renshaw D.
AU - Henderson R.
AU - Bonnist E.
PY - 2008
SP - 171
EP - 176
DO - 10.5220/0001061301710176