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Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics and National Academy of Sciences of Ukraine

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1 Papers

Development of Multi-parameter Analyser based on Electrochemical Urea Biosensors and Electrolyte Electrodes for Monitoring of Hemodialysis Patients

Topics: Biomedical Instrumentation; Biomedical Equipment; Biomedical Sensors; Health Monitoring Devices; Biomaterials

Authors: Julija Razumiene ¹ , Vidute Gureviciene ¹ , Marius Dagys ¹ , Ieva Sakinyte ¹ , Algimantas Jonuska ¹ , Laurynas Rimsevicius ² , Svitlana Marchenko ³ and Alexey Soldatkin ³

Affiliations: ¹ Institute of Biochemistry and Vilnius University, Lithuania , ² Nephrology Centre and Vilnius University, Lithuania , ³ Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics and National Academy of Sciences of Ukraine, Ukraine

Digital Object Identifier: 10.5220/0005814100930101

Proceedings of BIODEVICES 2016/BIOSTEC, ISBN: 978-989-758-170-0

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The idea of developing multi-parameter urea analyser comprising urea, Na+ and K+ selective electrodes has been considered. For this purpose the urea biosensors based on urease and recombinant urease working in amperometric and potentiometric way were developed. The working parameters of both urea biosensors were studied and optimized. Possibilities of real samples analysis using the developed biosensors were shown by measuring urea concentrations in blood dialysate taken from patients with renal failure. Both the potentiometric and the amperometric biosensors demonstrated high degree of signal reproducibility (the relative standard deviation of responses did not exceed 5 %). Change of sodium and potassium concentrations during blood hemodialysis is dangerous life-threatening condition and their monitoring is an important feature of point-of-care analyser. For this purpose high integrity commercial Na+ and K + selective electrodes were analysed and our own signal amplification and processing system proposed.

Related Ontology Subjects/Areas/Topics (10)

Biomaterials	Biomedical Instruments and Devices	Health Monitoring Devices
Biomedical Engineering	Biomedical Sensors	Human-Computer Interaction
Biomedical Equipment	Devices	Physiological Computing Systems
Biomedical Instrumentation