Yield Stability of Some Aloe Vera Clone

Hidayat

Postgraduate Program, Tanjungpura University, Pontianak, Indonesia

Keywords: Aloe vera, Stability, Yield.

Abstract: Research conducted in the field that was in Pontianak, Jl. Reformasi, Universitas Tanjungpura. Research on

third year using peat soil origin Rasau Jaya I, II and III. Observations were made on several properties such

as the increase in the number of leaves, the weight of the harvest (g), the width of the leaves (cm) and the

number of harvestable leaves. Observation results and components of yield is done to facilitate the

assessment of some of the clones produced.

1 INTRODUCTION

Total aloe vera harvest in Pontianak City in 2011

amounted to 460,000 m2 with total production of

7,360,000 kg with an average production of 16 kg /

m2. In North Pontianak Subdistrict, aloe vera

harvest area in 2010 amounted to 120,000 m2 with

total production of 478,800 kg or average production

of 3.99 kg / m2 (BPS Pontianak, 2011). Most of the

aloe vera plant is cultivated in peatlands.

Peat is a type of soil formed from the

accumulation of plant remnants that are half-

decomposed, therefore the content of organic matter

is high. Peat is defined as a material or organic

material buried naturally in a state of excessive wet,

incompressible and not or only slightly underwent.

Peat is organic soil, but does not mean organic soil is

peat soil (Noor, 2007). Generally peat soils react

sourly, so plants will be exposed to environmental

stresses that blindly disturbed plant growth.

The application of physical and chemical

mutagenes to somaclonal aloe vera is studied in

order to increase the frequency of somaclonal

diversity (Espina et al., 1991). Ethyl methane

sulfonate (EMS) is one type of alkylating agents that

effectively induces mutations in various organisms

(Fishbein, et al., 1970). EMS can also be used for

plant mutagenesis, but this report has not mentioned

the types of somklonal diversity formed by the

treatment (Epp, 1986) thus need to be studied

somaclonal diversity caused by the provision of

EMS especially on aloe vera.

The diversity in the resulting plants was

observed, then evaluated in the field. This evaluation

is important to determine whether the resulting

clones are resistant to acidity, how potential yields

are among the clones, the morphological changes of

how they occur, the answer to this question will be

the selection criteria for producing aloe vera clones

that tolerate acidity stress.

Aloe vera plants that allegedly originated from

the Canary islands in the west of Africa have been

known as medicine and cosmetics since centuries

ago recorded The Egyptian Book of Remendies. In

the days of Cleopatra, aloe vera is used for beauty

materials as a skin moisturizer. Pharmacy use was

first performed by the Samaritans approximately

1750 M. In China aloe vera is used as a traditional

medicine by drinking the liquid in order to cleanse

the organs of the body of the disease. Allegedly aloe

vera into Indonesia around the 17th century that

originally as an ornamental plant (Sumarno, 2002).

Aloe vera extract is a lot of benefits for health.

Utilization of aloe vera as a beverage is very

beneficial for health, among others, to maintain

kidney function, heart muscle, lower blood sugar

level, improve the immune system lowers blood

cholesterol levels, reduces symptoms of rheumatism,

canker sores, bleeding gums, sore throat digestive

tract, asthma, symptoms of dengue fever, deep heat,

reducing acne, and can be slimming (PT Botani

Tropical Lestari and Biology Department FMIPA-

UI, 2002). Constraints faced in the expansion of aloe

vera cultivation in peatlands is the likelihood of

seeds that resist stress acidity.

Efforts to obtain aloe vera seeds resistant to

environmental stress, free of pests and diseases can

be done through network cultivation. The success of

174

Hidayat, .

Yield Stability of Some Aloe Vera Clone.

DOI: 10.5220/0009899800002480

In Proceedings of the International Conference on Natural Resources and Sustainable Development (ICNRSD 2018), pages 174-181

ISBN: 978-989-758-543-2

tissue cultivation in the form of clonal opens

opportunities for genetic improvement of aloe vera

plant. The occurrence of deviation of the nature

(offtype) called somaclonal variation in seeds of

tissue culture can increase the genetic variability of

plants (Larkin, 1981 in Israeli et al., 1991).

The availability of sufficient genetic diversity is

needed to support the success of the selection

program. This report is a fact which shows that the

occurrence of somaclonal diversity in tissue culture

seeds can be utilized for genetic improvement of

plants.

The study of EMS on Cavendish banana and

banana Kepok Kuning resulted in the response of

EMS on Cavendish banana culture on growing

percentage, shoot number and shoot length

decreased in straight line with increasing of EMS

concentration level and morphological change that

was formed explant rate indicating mutation

(Hidayat , 2002, 2004).

The study of the use of EMS 5 on Aloe vera by

Cornelia (2003), showed faster growth time than

without EMS, and 0.05% concentration level. which

has created diversity. The results showed that there

was somaclonal diversity at the concentration level

ranging from 0.05% and increasing in line with the

increase of the EMS concentration level. The best

concentration gives rise to somaclonal diversity at

the level of 0.10% then will cause dead eksplan

(Hidayat, 2006). The resulting planlet was further

acclimatized to produce seeds and observed

morphological changes. The role of the environment

is enormous for the growth and development of

plants, a growing environment that grips growth and

growth of plants.

Environment is a potential environmental factor

unfavorable to the life of the creature in its general

(Levitt, 1980). In general, environmental stress is

grouped into 2, namely: (1). Biotic cohesion consists

of: a) intra-species and inter-species competition, b)

infection by pests and diseases. (2). Abiotic stresses

are a) temperatures (high and low), b) water

(advantages and disadvantages), c) radiation

(ultraviolet, infrared, visible light and ionizing

radiation), d) chemicals (salts, gases, and pesticides)

, e) wind, and f) sound.

Environmental stresses on peat soils may include

soil acidity (pH <5.0), excess water (puddle height),

pyrite, and salinity. Areas like this are widely

available outside of Java.

Indonesia's swamp land is 33.4 million ha spread

over Sumatra, Kalimantan, Sulawesi and Irian Jaya.

20.1 million ha is tidal swamp land and 13.3 million

ha of nontidal swamps. Peat typology covers an area

of 11.0 million ha, acid sulphate of 6.7 million ha

and saline / somewhat saline 0.4 million ha

(Balitbangtan Pangan, 1992 in Hidayat, 2009). Thus

peat soil type is wide enough to be utilized.

Efforts to improve peatland productivity can be

done through improved soil fertility by fertilizing

and improving the soil (calcification) and other

businesses such as ash, burning residue, wood

processing plant waste, ash (palm rest residue),

volcanic ash, and marine mud.

An effort would require large expenses and very

large material inputs (eg to raise the soil pH of a

single cake required 50 tons / ha of lime) so that it is

less efficient, one of the alternatives offered is the

search and assembling of resistant and suitable

varieties / clones in the local environment needs

attention.

Patent Search Description Based on patent search

results on some patent sites such as www.uspto.gov,

www.jpo-miti.go, www.ipaustralia.gov.au., Www.

patents1.ic.gc.ca/ intro-e-html, and www.european-

patent-office.org/ espacenet / info / index.h,

www.delphio.com have not found any patent

regarding making aloe vera clones. Several studies

are directed at utilization of Aloe vera leaf bleed.

Some search results are presented in Table 1.

Additional Some Patent Search Results About

Aloe Vera: 7,205,012 B1 = Scar reducing and

massage emollient by Wendy L. Hill, 4758 Appleton

St., San Diego, Calif. 92117 (US) Filed on Feb. 25,

2005, as Appl. No. 11/65,661. Int. Cl. A01N 65/00

(2006.01) U.S. Cl. 424—764 [424/765; 424/745;

424/744] 2.

Claims: 1. A method of making a scar reduction

emollient, said method comprising the steps of:

Mixing together calendula flowers, chamomile

flowers, comfrey leaf, rose petals, rosemary and rose

geranium to form a herb mixture; Positioning the

herb mixture in a cooking vessel and covering the

herb mixture with almond oil and olive oil and

heating the cooking vessel at a temperature below

the boiling point of the olive and almond oils to

define a heated mixture; Straining the heated

mixture through cheesecloth to define a strained oil;

Melting beeswax and mixing together said beeswax

with shea butter, lavender oil, Ylang Ylang oil, Aloe

vera gel, jojoba oil, wheat germ oil, evening

primrose oil and said strained oil to define said

emollient; and Positioning said emollient in at least

one container.

Yield Stability of Some Aloe Vera Clone

175

Table 1: Search Results of Aloe Vera Patent Document

Source : www.uspt.gov., March 11, 2010 and April 20, 2014

2 RESEARCH METHODS

Research conducted in Pontianak Jl. Reform. The

study used Randomized Block Design on the basis

of observed morphological diversity such as aloe

vera plant height of approximately 24 months as the

basis of grouping into five groups and five

treatments based on origin in laboratory ie EMS

concentration. The third year research is arranged in

serial / multi location with 2 series based on the

origin of peat land taken by Rasau Jaya I and III.

Materials research in the form of aloe vera plants

from previous studies aged 24 months, chicken

manure , fertilizer NPK trademark Ponska. The

No. No. Patent

Total

claim

Description patent Inventor Year

US 7,196,072 B2

High molecular weight

polysaccharide fraction

from Aloe vera with

immunostimulatory

activity

David Stanley Pasco,

Oxford, Miss. (US);

Nirmal Derek Ceri Pugh,

Oxford, Miss. (US);

Mahmoud ElSohly,

Oxford, Miss. (US); and

Samir Ross, Oxford, Miss.

(US)

Assigned to University of

Mississippi, University,

Miss. (US)

PCT Pub. No.

WO02/03999,

PCT Pub. Date

Jan. 17, 2002.

US 7,262,224 B2

Cosmetic rejuvenating

and healing product,

method of its manufacture

and uses thereof

Myong Hun Chong,

Arlin

ton, Tex. (US)

Hanna Isul Skin Therapy,

Inc., Fort Worth, Tex.

(US)

Prior

Publication US

2003/0223953

A1, Dec. 04,

2003

US 7,332,151 B2

Liquid animal hoof

conditioner

Ben Ray Yoder, N. 4825

Highway 104, Brodhead,

Wis. 53520 (US)

Prior

Publication US

2005/0266103

A1, Dec. 01,

2005

US 7,198,779 B2

Compositions for the

relief of xerostomia and

the treatment of

associated disorders

Ana Rifa Piñol, Martorell

(Spain); and Montserrat

Mata Moliner, Sant

Sadurni d'Anoia (Spain

PCT Pub. No.

WO03/028699,

PCT Pub. Date

Apr. 10, 2003.

S 7,252,846 B2

Topical composition and

method for the treatment

and prophylaxis of dermal

irritations

Raied Dinno, 727 South

Ave., Weston, Mass.

02439 (US)

Prior

Publication US

2005/0266094

A1, Dec. 01,

2005

7,205,012 B1

Scar reducing and

massage emollient

Wendy L. Hill, 4758

Appleton St., San Diego,

Calif. 92117 (US)

Filed on Feb.

25, 2005, as

Appl. No.

11/65,661.

US 7,329,421 B2

Process of Manufacturing

Clear Juice From The

Leaves of The Aloe Vera

Plant

Agashe Mandar

Dnyaneshwar, 242,

Shaniwar Peth, Pune 411

030 (India)

Prior

Publication US

2006/0134238

A1, Jun. 22,

2006

ICNRSD 2018 - International Conference on Natural Resources and Sustainable Development

176

necessary tools are farming tools, ATK, balance

sheet, camera, and so on.

Soil processing in the form of cleaning the land

to put polybags from weeds and the remnants of

weeds, stumps and so on. Aloe vera plant is

maintained since the previous research, maintenance

in the form of weeding, fertilization, and pest control

is done regularly and intensively.

The study used a Randomized Block Design

consisting of 5 Aloe vera clones: E4, E5, E6, E7,

and E8, as treatment and 2 sets of research ie 2 sets

of research on peat soil origin Rasau Jaya I and II 2

set of research on peat soil origin Rasau Jaya I and

II. The research observed were :

1. The amount of leaf, observed from the beginning

to the end of the study.

2. The thickness of the leaf by measuring the

thickness in the middle of the leaf

3. The length of the leaf thrower, measured from

the base of the leaf to the tip of the leaf

4. Total Weight (g) leaf sheath that can be

harvested

5. Weight per leaf bower is harvested, done by

weighing the leaves are harvested

The research data of the combined variance

analysis is then tested for power yield stability.

3 RESULTS AND DISCUSSION

Research data on each series of research then

analyzed the variance by using Randomized Block

Design for each series of research, then tested the

combined variance. It turns out all research variables

showed the inettraction genotype (clone) and

Environment (GxE) have a very real effect, that aloe

vera clones have a diversity that is environmentally

influenced. Further analysis of yield stability

obtained from aloe vera plants. The adaptability and

stability results of the length component of aloe vera

bark can be seen in Table 4. Looks Clone E8 is

relatively stable compared to other clones.

Clone E4 is relatively stable, but no single clone

is capable of general adaptation. Looks older age of

aloe vera plant is the 3rd year (360 days) thick bark

higher. Table 5 shows only a relatively stable E4

clone, whereas general adaptability does not contain

a capable clone. It can be seen that the thickness of

harvest bark on peat soil from Rasau Jaya I is thicker

than that of the peat soil from Rasau Jaya III. This is

possible because Rasau Jaya I peatlands are

relatively more fertile compared to peatlands from

Rasau Jaya III.

All clones were able to convert to Rasau Jaya I,

II and III, but for Rasau Jaya II all clones were badly

adapted, clone E 7 was stable. The ability to grow is

influenced by environmental factors such as soil

fertility and soil acidity.

Figure 1: (a) Measurement of the leaf length, (b) Weight,

4 CONCLUSIONS

1. The peatlands from Rasau Jaya I are relatively

more fertile than those Rasau Jaya III and Rasau

Jaya II.

2. Aloe vera clones E4 and E7 have adaptability

and power stability results which is good to be

cultivated in two peat soils

3. The yield stability for each clone has not been

stable, so it is necessary multi-location

experiments are done again.

4. A multi-location test on Rasau Jaya I and III

peatlands is required to obtain better results, so

clones can be removed.

REFERENCES

Anonymous, 2004. Study of EMS Giving on Banana

Kepok Kuning Shoots. [Dissertation]. Research

Institute of Tanjungpura University. Pontianak.

Yield Stability of Some Aloe Vera Clone

177

Anonymous, 2006. Somaklonal Aloe Vera. [Dissertation].

Research Institute of Tanjungpura University.

Pontianak.

Anonymous, 2009. Characterization and Identification of

Aloe Vera Clones Breeding Mutation of Ethyl Methane

Sulfonate. [Dissertation]. Research Institute of

Tanjungpura University. Pontianak.

Anonymous, 2015. Aloe Vera Holds Acidity stress.

[Dissertation]. Research Institute of the Tiger.

Pontianak.

Anonymous. 2014. Several Clones of Aloe Vera Holds

Acidity Stress. [Dissertation]. Research Institute of

Tires. Pontianak.

Australia Intellectual Property. Retrieved from

http//www.ipaustralia.gov.au., [February 25, 2010;

April 20, 2014].

Canadian Patent Database. Retrieved fom

http//www.patents1.ic.gc.ca/intro-e-html [March 11,

2010; April 20, 2014].

Cornelia, 2003. Study Giving Ethyl Methane Sulfonate

Against Growth Eksplan Aloe Vera. Fakultas

Pertanian Universitas. Tanjungpura, Pontianak (not

published).

Delphion. Retrieved from http//www.delphio.com, [March

11, 2010; April 20, 2014]

Epp, M.D., 1986. Somaclonal Variation in Bananas: A

case Study with Fusarium Wilt. In G.J. Persley and

E.A. de Langhe (Eds.). Banana and Plantain in

Breeding Strategies. Australian Centre for

International Agricultural Research (ACIAR)

Proceedings, Australian Centre for International

Agricultural Research.pp. 140-50.

Espacenet. Retrieved from http://www.ep.espacenet.com.

[February 29, 2010; April 20, 2014].

Espino, R.R.C., Jamaluddin, S.H., Silayoi, B., dan

Nasution, E., 1991. Edible Fruit and Nut. In E.W.M.

Verhej and R.E.Coronet (Eds.). Plant Resources of

South East Asia. Pudoc. Wageningen. pp. 225-330.

European Patent Office. Retrieved from

http//www.epo.org/espacenet/info/index.h. [March 10,

2010; April 20, 2014].

Hidayat, 2003. Somaclonal Variations of Banana Shoots

Cavendish. Agrista. 7(1): 74-79.

Israeli, Y., Reuveni, O., dan Lahav, E., 1991. Qualitaive

Aspects of Somaclonal Variations in Bananas

Propagated by In-vitro Techniques. Scientia

Horticulture. 48: 71-88.

Japan Patent Office. Retrieved from http//www.jpo-

miti.go. [February 15, 2010; April 20, 2014]

Levitt, J., 1980. Responses of Plants to Environmental

Stresses, Vol. II.

Levitt, J., 2015. Water, Radiation, Salt, and Other

Stresses, Academic Press. New York. p. 607.

Monsanto. Retrieved from http://www.monsanto.com,

[March 11, 2010; April 20, 2014]

P.T. Botany Tropical Lestari and Biology Department

FMIPA-UI. 2002. At a Kavera (leflet). Jakarta.

Singh, R.K., dan Chaudary, B.D., 1979. Biometrichal

Methods in Quantitative Genetic Análisis. Kalyani

Publishers. Ludhiana, New Delhi. p. 304.

Sumarno, 2002. Aloe Vera Development Program in

Indonesia. Aloe vera. 1(1): 6-10.

Syngenta Global. Retrieved from

http://www.syngenta.com. [March 11, 2010; April 20,

2014]

United States Patent and Trademark Office. Retrieved

from http://www. Uspto.gov/patft/index.html. [March

11, 2010; April 20, 2014].

ICNRSD 2018 - International Conference on Natural Resources and Sustainable Development

178

APPENDIX

List of supplementary data in this study are

presented in tables as following:

Table 2: Class 424 Drug, Bio-Affecting and Body Treating Compositions.

1.11 Radionuclide or int; adjuvant or carrier compositions; intermediate or preparatory compositions

1.13 In aerosol, fine spray, effervescent, pressurized fluid, vapor or gas, or complete composition

1.17 Attached to or within viable or inviable whole micro-organism, cell, virus, fungus or specified sub-cellular structure thereof

(e.g., platelet, red blood cell)

1.21 Molecular bilayer structure (e.g., vesicle, liposome)

1.25 Dissolving or eluting from solid or gel matrix (e.g., capsule, tablet)

1.29 Coated, impregnated, or colloidal particulate (e.g., microcapsule, micro-sphere, micro-aggregate, macro-aggregate)

1.33 Delivery to active site involves particle dissolving, degrading, or otherwise releasing of radionuclide

1.37 Radionuclide or intended radionuclide in an organic compound

1.41 Attached to lymphokine, cytokine, or other secreted growth regulatory factor, differentiation factor, or intercellular mediator

specific for a hematopoietic cell (e.g., interferon, interleukin, macrophage factor, colony stimulating factor, erythropoietin);

derivative thereof

1.45 Attached to cyclopentano-hydrophenanthrene (e.g., cholesterol, bile acid, steroids, cholane), hormone, or neurotransmitter, or

other secreted growth regulatory factor, differentiation factor, or intercellular mediator (e.g., t3, t4, insulin, human chorionic

gonadotropin, intragonadal regulatory protein, mullerian inhibiting substance, inhibin, epidermal growth factor, nerve growth

factor, dopamine, norepinephrine); derivative thereof

1.49 Attached to antibody or antibody fragment or immunoglobulin; derivative

1.53 Attachment via an added element (e.g., bifunctional compound or coordinate, coupling agent, spacer compound, bridging

compound, conjugated chelate)

1.57 Attached to antigen or hapten; derivative thereof

1.61 In an inorganic compoun

1.65 In an organic compoun

1.69

Attached to peptide or protein of 2+ amino acid units (e.g., dipeptide, folate, fibrinogen, transferrin, sp. Enzymes); derivative thereof

1.73 Attached to carbohydrate compound; derivative thereof (e.g., dna, nucleotide, nucleoside, sugar, starch, tannin, saccharide,

polysaccharide, cellulose, o-, n- and s-glycoside, vitamin b12)

1.77 Phosphorus-containing organic compoun

1.81 Nonmetal radionuclide or intended radionuclide (e.g., carbon)

1.85 Halogen

1.89 Fluorine

9.1 In vivo diagnosis or in vivo testing

9.2 Testing efficacy or toxicity of a compound or composition (e.g., drug, vaccine, etc.)

9.3 Magnetic imaging agent (e.g., nmr, mri, mrs, etc.)

9.31 Clay or zeolite containing

9.32 Particle containing a transition, actinide, or lanthanide metal (e.g., hollow or solid particle, granule, etc.)

9.321 Liposome

9.322 Polymer containing (e.g., polypeptide, synthetic resin, etc.)

9.323 Metal is paramagnetic

9.33 Nitroxide or nitroxide containing

9.34 Polypeptide attached to or complexed with the agent (e.g., protein, antibody, etc.)

9.341 The region of the imaging agent responsible for binding to an in vivo target or the region of the target responsible for binding to

the agent is specifically recited functionally or as a sequence of amino acids, carbohydrate residues, or nucleic acids

9.35 Carbohydrate or derivative thereof attached to or complexed with the agent

9.351 The region of the imaging agent responsibile for binding to an in vivo target or the region of the target responsible for binding to

the agent is specifically recited functionally or as a sequence of amino acids, carbohydrate residues, or nucleic acids

9.36 Transition, actinide, or lanthanide metal containing

9.361 Heterocyclic compound is attached to or complexed with the metal

9.362 Porphyrin or derivative thereof

9.363 Hetero ring contains at least eight members

9.364 Polyamino-polycarbonyl moiety attached to or complexed with the metal

9.365 Contains at least one -c(=o)-n- group

9.37 Imageable halogen containing

9.4 X-ray contrast imaging agent (e.g. computed tomography, angiography, etc.)

9.41 Barium containing

9.411 Polymer containing (e.g., polypeptide, synthetic resin, etc.)

9.42 Transition, actinide, or lanthanide metal containing

9.43 * Carbohydrate or derivative thereof attached to or complexed with the agent

257.1* Escherichia (e.g., escherichia coli, etc.)

Yield Stability of Some Aloe Vera Clone

179

Table 3: Stability Result of Number of Harvest of Aloe Vera Clone

General average = 22,58

Note : RJ I

= Rasau Jaya I first year; RJ III

= Rasau Jaya III first year ;

RJ I

= Rasau Jaya I Second year ; RJ III

Rasau Jaya III Second year

RJ II

= Rasau Jaya II first year ; RJ I

= Rasau Jaya I first year;

RJ II

= Rasau Jaya II third year; RK = rank

Table 4: Stability of Wide Harvest Results of Aloe Vera Clone.

General average= 10,14

Note: RJ I= Rasau Jaya I ; RJ III= Rasau Jaya III; RJI* = konversi data RJ I data conversion; RJ III*= Conversion of data

Rasau Jaya; RK = rank

Table 5: Stability of Long Leaf Harvesting of Aloe Vera Clone.

General average = 30,11

Note: RJ I= Rasau Jaya I ; RJ III= Rasau Jaya III; RJI* = konversi data RJ I data conversion; RJ III*= Conversion of data

Rasau Jaya; RK = rank

Table 6: Stability of Width Leaf Harvesting of Aloe vera Clone

General average = 2.57

Note: RJ I= Rasau Jaya I ; RJ III= Rasau Jaya III; RJI* = konversi data RJ I data conversion; RJ III*= Conversion of data

Rasau Jaya; RK = rank

Clone RJ I

RK RJI

RK RJII

RK RJ III

RK RJ I

RK RJII

10.60 3 8.93 5 15,25 2 11.67 3 9.67 5 41.25 5 45.75 2

10.47 4 8.93 4 13.33 5 13.07 1 11.53 1 42.25 2 40.00 5

10.20 5 9.00 3 14.92 3 12.73 2 11.13 3 41.50 4 44.75 3

11.73 1 9.07 2 14.08 4 10.87 5 11.33 2 55.50 1 42.25 4

11.60 2 9.80 1 15.92 1 11.47 4 9.77 4 42.25 3 47.75 1

Tot. 54.60 45.73 73,50 59.80 53.43 222.7 220.5

. 10.92 9.15 14.70 11.96 10.69 44.55 44.10

Clone RJ I RK RJI* RK ᵡ

RJ III RK RJ III* RK ᵡ

6.99 3 35.00 4 96.00 6.07 3 30.51 2 10.29

7.20 2 35.81 1 0.00 6.49 1 30.48 3 43.20

7.36 1 35.01 2 10.29 6.11 2 30.52 1 0.00

6.65 5 35.01 3 43.20 6.05 4 25.52 5 163.64

6.82 4 34.98 3 163.64 5.77 5 30.48 4 96.00

Total 35.02 30.49 5 27.71

Avera

e 7.00 6.10

Clone RJ I RK RJI* RK ᵡ

RJ III RK

III*

RK ᵡ

46.48 1 215.66 1 0.00 35.65 5 202.57 1 0.00

41.77 4 215.65 3 43.20 35.98 4 202.54 5 163.64

43.40 3 215.64 4 96.00 36.86 2 202.55 4 96.00

39.44 5 215.64 5 163.64 36.14 3 202.57 2 10.29

44.57 2 215.66 2 10.29 37.64 1 202.56 3 43.20

Total 215.66 182.27

Avera

e 43.13 36.45

Clone RJ I RK RJI* RK ᵡ

RJ III RK RJ III* RK ᵡ

1.62 4 8.72 1 0.00 1.45 3 8.49 2 10.29

1.61 5 8.70 4 96.00 1.42 4 8.75 1 0.00

1.64 2 8.69 5 163.64 1.50 1 8.39 4 96.00

1.65 1 8.72 2 10.29 1.41 5 8.47 3 43.20

1.63 3 8.71 3 43.20 1.48 2 8.24 5 163.64

Total 8.14 7.26

Avera

e 1.63 1.45

ICNRSD 2018 - International Conference on Natural Resources and Sustainable Development

180

Table 7: Stability of Weight Results per Harvesting of Aloe Vera Clone.

General average = 494,12

Note: RJ I= Rasau Jaya I ; RJ III= Rasau Jaya III; RJI* = konversi data RJ I data conversion; RJ III*= Conversion of data

Rasau Jaya; RK = rank

Clone RJ I RK RJI* RK ᵡ

RJ III RK RJ III* RK ᵡ

631.50 3 7381.25 1 0.00 561.67 2 6370.95 2 10.29

611.75 5 7381.23 3 43.20 419.27 4 6370.96 1 0.00

644.17 2 7381.22 4 96.00 625.33 1 6370.94 3 43.20

625.58 4 7381.22 5 163.64 396.38 5 6370.92 5 163.64

650.58 1 7381.24 2 10.29 439.27 3 6370.94 4 96.00

Total 3163.58 4089.45

Avera

e 632.72 817.89

Yield Stability of Some Aloe Vera Clone

181