Microfluidic Prototype of a Lab-on-Chip Device for Lung Cancer Diagnostics

Dalila Vieira, Filipa Mata, Ana Moita, António Moreira

Abstract

Cell sorting for disease diagnostics is often achieved by fluorescence based identification of specific markers. However, in lung cancer diagnostics, cytological analysis of pleural fluids is not always reliable and immunofluorescence essays demand for specific sample preparation. Hence, this paper addresses the development of a microfluidic device for lung cancer diagnostics which infers on the potential of a diagnosis based on analysing the cell deformability (stiffness) that alters the rheological properties and consequently the flow characteristics. Cell deformability will be induced by external actuation. Electrowetting is used to transport the samples in an open configuration system using microdroplets. Effects of the test chip configuration, sample physico-chemical properties and potential adsorption mechanisms are discussed. Wettability plays here a vital role in the sample transport and in the diagnostic method to be tested. Hence, an innovative approach is presented, the 3D Laser Scanning Fluorescence Confocal Microscopy (3D-LSCFM) to provide a detailed reconstruction of the surface topology at the liquid-solid interface region thus allowing contact angles measurement with high spatial resolution.

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Paper Citation


in Harvard Style

Vieira D., Mata F., Moita A. and Moreira A. (2017). Microfluidic Prototype of a Lab-on-Chip Device for Lung Cancer Diagnostics . In Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017) ISBN 978-989-758-216-5, pages 63-68. DOI: 10.5220/0006252700630068


in Bibtex Style

@conference{biodevices17,
author={Dalila Vieira and Filipa Mata and Ana Moita and António Moreira},
title={Microfluidic Prototype of a Lab-on-Chip Device for Lung Cancer Diagnostics},
booktitle={Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)},
year={2017},
pages={63-68},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006252700630068},
isbn={978-989-758-216-5},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)
TI - Microfluidic Prototype of a Lab-on-Chip Device for Lung Cancer Diagnostics
SN - 978-989-758-216-5
AU - Vieira D.
AU - Mata F.
AU - Moita A.
AU - Moreira A.
PY - 2017
SP - 63
EP - 68
DO - 10.5220/0006252700630068