A Portable Sensing System for Impedance based Detection of Biotoxin Substances

V. I. Ogurtsov, K. Twomey, J. Pulka

Abstract

The study describes the development of a portable autonomous biosensing platform for impedance based detection of biotoxin substances. The platform implements a label-free approach, which is based on detection of the biosensor interfacial changes due to a bio recognition reaction. The interfacial changes are sensed by means of Electrochemical Impedance Spectroscopy in a frequency range from 10 Hz to 100 kHz. The platform comprises of an electrochemical biosensor, portable low-noise mix signal hardware and associated software incorporating signal processing algorithms for extraction biotarget concentration from the biosensor response. The biosensor is realized as an on-chip package-free three electrode micro electrochemical cell consisted of a counter electrode (CE), a reference electrode (RE) and a working electrode (WE) patterned on a single silicon chip. WE represents an array of 40 um diameter gold disks with 400 m center-to-center distance, which were undergone of corresponding surface modification for antibody immobilisation. The developed system was validated by an example of T-2 toxin detection. Performed calibration in the range of 0 –250 ppm of T2 toxin concentrations confirmed that the system can provide successful detection of the toxin at the levels below 25 ppm.

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Paper Citation


in Harvard Style

Ogurtsov V., Twomey K. and Pulka J. (2017). A Portable Sensing System for Impedance based Detection of Biotoxin Substances . In Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017) ISBN 978-989-758-216-5, pages 54-62. DOI: 10.5220/0006169200540062


in Bibtex Style

@conference{biodevices17,
author={V. I. Ogurtsov and K. Twomey and J. Pulka},
title={A Portable Sensing System for Impedance based Detection of Biotoxin Substances},
booktitle={Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)},
year={2017},
pages={54-62},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006169200540062},
isbn={978-989-758-216-5},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)
TI - A Portable Sensing System for Impedance based Detection of Biotoxin Substances
SN - 978-989-758-216-5
AU - Ogurtsov V.
AU - Twomey K.
AU - Pulka J.
PY - 2017
SP - 54
EP - 62
DO - 10.5220/0006169200540062