μSmartScope: 3D-printed Smartphone Microscope with Motorized Automated Stage

Luís Rosado, João Oliveira, Maria João M. Vasconcelos, José M. Correia da Costa, Dirk Elias, Jaime S. Cardoso

Abstract

Microscopic examination is currently the gold standard test for diagnosis of several neglected tropical diseases. However, reliable identification of parasitic infections requires in-depth train and access to proper equipment for subsequent microscopic analysis. These requirements are closely related with the increasing interest in the development of computer-aided diagnosis systems, and Mobile Health is starting to play an important role when it comes to health in Africa, allowing for distributed solutions that provide access to complex diagnosis even in rural areas. In this paper, we present a 3D-printed microscope that can easily be attached to a wide range of mobile devices models. To the best of our knowledge, this is the first proposed smartphone-based alternative to conventional microscopy that allows autonomous acquisition of a pre-defined number of images at 1000x magnification with suitable resolution, by using a motorized automated stage fully powered and controlled by a smartphone, without the need of manual focus of the smear slide. Reference smears slides with different parasites were used to test the device. The acquired images showed that was possible to visually detect those agents, which clearly illustrate the potential that this device can have, specially in developing countries with limited access to healthcare services.

References

  1. Arpa, A., Wetzstein, G., Lanman, D., and Raskar, R. (2012). Single lens off-chip cellphone microscopy. pages 23- 28. IEEE.
  2. Cybulski, J. S., Clements, J., and Prakash, M. (2014). Foldscope: Origami-Based Paper Microscope. PLoS ONE, 9(6):e98781.
  3. Dolgin, E. (2015). Portable pathology for Africa. IEEE Spectrum, 52(1):37-39.
  4. Krotkov, E. (1988). Focusing. International Journal of Computer Vision, 1(3):223-237.
  5. Liu, X., Wang, W., and Sun, Y. (2007). Dynamic evaluation of autofocusing for automated microscopic analysis of blood smear and pap smear. Journal of microscopy, 227(1):15-23.
  6. Pirnstill, C. W. and Cot, G. L. (2015). Malaria Diagnosis Using a Mobile Phone Polarized Microscope. Scientific Reports, 5:13368.
  7. Quinn, J., Andama, A., Munabi, I., and Kiwanuka, F. (2014). Automated Blood Smear Analysis for Mobile Malaria Diagnosis. In Mobile Point-of-Care Monitors and Diagnostic Device Design, Devices, Circuits, and Systems.
  8. Rosado, L., Costa, J. M. C. d., Elias, D., and Cardoso, J. S. (2016). A Review of Automatic Malaria Parasites Detection and Segmentation in Microscopic Images. http://www.eurekaselect.com, 14(1):11-22.
  9. Shih, L. (2007). Autofocus survey: a comparison of algorithms. In Electronic Imaging 2007, pages 65020B65020B. International Society for Optics and Photonics.
  10. Smith, Z. J., Chu, K., Espenson, A. R., Rahimzadeh, M., Gryshuk, A., Molinaro, M., Dwyre, D. M., Lane, S., Matthews, D., and Wachsmann-Hogiu, S. (2011). Cell-Phone-Based Platform for Biomedical Device Development and Education Applications. PLoS ONE, 6(3):e17150.
  11. Sun, Y., Duthaler, S., and Nelson, B. J. (2005). Autofocusing algorithm selection in computer microscopy. In 2005 IEEE/RSJ International Conference on Intelligent Robots and Systems, pages 70-76. IEEE.
  12. Switz, N. A., D'Ambrosio, M. V., and Fletcher, D. A. (2014). Low-Cost Mobile Phone Microscopy with a Reversed Mobile Phone Camera Lens. PLoS ONE, 9(5):e95330.
  13. Tenenbaum, J. M. (1970). Accommodation in computer vision. phd dissertation. Technical report, DTIC Document.
  14. Tseng, D., Mudanyali, O., Oztoprak, C., Isikman, S. O., Sencan, I., Yaglidere, O., and Ozcan, A. (2010). Lensfree microscopy on a cellphone. Lab on a Chip, 10(14):1787-1792.
  15. Utzinger, J., Becker, S. L., Knopp, S., Blum, J., Neumayr, A. L., Keiser, J., and Hatz, C. F. (2012). Neglected tropical diseases: diagnosis, clinical management, treatment and control. Swiss Medical Weekly, 142:w13727.
  16. Wakerly, M. (2012). Usb serial for android.
  17. WHO, W. H. O. (1991). Basic malaria microscopy.
  18. Zachary, G. (2015). Technology alone won't improve health in Africa [Spectral lines]. IEEE Spectrum, 52(1):7-7.
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Paper Citation


in Harvard Style

Rosado L., Oliveira J., João M. Vasconcelos M., M. Correia da Costa J., Elias D. and S. Cardoso J. (2017). μSmartScope: 3D-printed Smartphone Microscope with Motorized Automated Stage . In Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017) ISBN 978-989-758-216-5, pages 38-48. DOI: 10.5220/0006155800380048


in Bibtex Style

@conference{biodevices17,
author={Luís Rosado and João Oliveira and Maria João M. Vasconcelos and José M. Correia da Costa and Dirk Elias and Jaime S. Cardoso},
title={μSmartScope: 3D-printed Smartphone Microscope with Motorized Automated Stage},
booktitle={Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)},
year={2017},
pages={38-48},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0006155800380048},
isbn={978-989-758-216-5},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 10th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2017)
TI - μSmartScope: 3D-printed Smartphone Microscope with Motorized Automated Stage
SN - 978-989-758-216-5
AU - Rosado L.
AU - Oliveira J.
AU - João M. Vasconcelos M.
AU - M. Correia da Costa J.
AU - Elias D.
AU - S. Cardoso J.
PY - 2017
SP - 38
EP - 48
DO - 10.5220/0006155800380048