microRNA detection with an active nanodevice FoF1-ATPase

Yao-Gen Shu, Zhong-Can Ou-Yang

Abstract

We have developed a novel nanodevice constituted with a rotary motor and a ``battery'', FoF1-ATPase and chromatophore. The latter can be recharged enough energy by light so that the former can rotates at about 1000 r.p.m for more than one hour. If the nanodevice is captured by a target such as miRNA and processively rotate for 30 minutes, the number of targets will be amplified by 100000 ATP molecules. This method has potential to be developed into an ultrasensitive biosensor to detect low expressed targets such as miRNA.

References

  1. Abrahams, J. P., Leslie, A. G. W., Lutter, R., and Walker, J. E. (1994). Structure at 2.8a° resolution of F1- ATPase from bovine heart mitochondria. Nature, 370:621-628.
  2. Boyer, P. D. (1997). The ATP synthase-a splendid molecular machine. Annu. Rev. Biochem., 66:717-749.
  3. Cheng, J., Zhang, X. A., Shu, Y. G., and Yue, J. C. (2010). FoF1-ATPase activity regulated by external links on ß subunits. Biochem. Biophys. Res. Commun., 391:182- 186.
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Paper Citation


in Harvard Style

Shu Y. and Ou-Yang Z. (2016). microRNA detection with an active nanodevice FoF1-ATPase . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 166-169. DOI: 10.5220/0005692301660169


in Bibtex Style

@conference{biodevices16,
author={Yao-Gen Shu and Zhong-Can Ou-Yang},
title={microRNA detection with an active nanodevice FoF1-ATPase},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)},
year={2016},
pages={166-169},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005692301660169},
isbn={978-989-758-170-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)
TI - microRNA detection with an active nanodevice FoF1-ATPase
SN - 978-989-758-170-0
AU - Shu Y.
AU - Ou-Yang Z.
PY - 2016
SP - 166
EP - 169
DO - 10.5220/0005692301660169