Low Cost 3D-Printed Biosensor Arrays for Protein-based Cancer Diagnostics based on Electrochemiluminescence

James F. Rusling, Karteek Kadimisetty, Spundana Malla, Gregory W. Bishop, Jennifer E. Satterwhite-Warden

Abstract

Development and fabrication of bioanalytical devices by 3D printing offers revolutionary new routes to low cost clinical diagnostic devices for molecular measurements. Relevant to future protein-based cancer diagnostics, we describe and review here our recent development of prototype protein immunoarray devices using desktop Fused Deposition Modeling (FDM) and stereolithographic 3D printers. All these system feature sensitive electro-optical detection by a method called electrochemiluminescence (ECL). Our first 3D-printed immunoarray features screen-printed sensors in which manual manipulations enable gravity flow reagent delivery for measurement of 3 proteins at detection limits of 0.3 to 0.5 pg/mL. ECL detection is achieved in an open channel on integrated disposable screen-printed sensor elements. We then address the issue of printing and processing optically clear plastic using a stereolithographic printer to build a closed ECL detection chamber. Finally, we describe a prototype 3D-printed microprocessor-controlled enclosed microfluidic ECL immunoarray featuring reagent reservoirs, micropumps and clear plastic detection chamber with printed nanowells for ECL emission.

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Paper Citation


in Harvard Style

Rusling J., Kadimisetty K., Malla S., Bishop G. and Satterwhite-Warden J. (2016). Low Cost 3D-Printed Biosensor Arrays for Protein-based Cancer Diagnostics based on Electrochemiluminescence . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 17-22. DOI: 10.5220/0005649000170022


in Bibtex Style

@conference{biodevices16,
author={James F. Rusling and Karteek Kadimisetty and Spundana Malla and Gregory W. Bishop and Jennifer E. Satterwhite-Warden},
title={Low Cost 3D-Printed Biosensor Arrays for Protein-based Cancer Diagnostics based on Electrochemiluminescence},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)},
year={2016},
pages={17-22},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005649000170022},
isbn={978-989-758-170-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)
TI - Low Cost 3D-Printed Biosensor Arrays for Protein-based Cancer Diagnostics based on Electrochemiluminescence
SN - 978-989-758-170-0
AU - Rusling J.
AU - Kadimisetty K.
AU - Malla S.
AU - Bishop G.
AU - Satterwhite-Warden J.
PY - 2016
SP - 17
EP - 22
DO - 10.5220/0005649000170022