Robustness to Sub-optimal Temperatures of the Processes of Tsr Cluster Formation and Positioning in Escherichia Coli

Teppo Annila, Ramakanth Neeli-Venkata, Andre S. Ribeiro

Abstract

Clustering and positioning of chemotaxis-associated proteins are believed to be essential steps for their proper functioning. We investigate the robustness of these processes to sub-optimal temperatures by studying the size and location of clusters of Tsr-Venus proteins in live cells. We find that the degree of clustering of Tsr proteins is maximal under optimal temperature. The data further suggests that the weakening of the clustering process in lower-than and higher-than optimal temperatures is not due to the same cause. Meanwhile, the location of the clusters is found to be weakly temperature independent, within the range tested. We conclude that while the clustering of Tsr is heavily temperature dependent, the localization is only weakly dependent, suggesting that the functionality of the proteins responsible for retaining Tsr-clusters at the cell poles, such as the Tol-Pal complex, is robust to suboptimal temperatures.

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Paper Citation


in Harvard Style

Annila T., Neeli-Venkata R. and Ribeiro A. (2016). Robustness to Sub-optimal Temperatures of the Processes of Tsr Cluster Formation and Positioning in Escherichia Coli . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 3: BIOINFORMATICS, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 137-141. DOI: 10.5220/0005647001370141


in Bibtex Style

@conference{bioinformatics16,
author={Teppo Annila and Ramakanth Neeli-Venkata and Andre S. Ribeiro},
title={Robustness to Sub-optimal Temperatures of the Processes of Tsr Cluster Formation and Positioning in Escherichia Coli},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 3: BIOINFORMATICS, (BIOSTEC 2016)},
year={2016},
pages={137-141},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005647001370141},
isbn={978-989-758-170-0},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 3: BIOINFORMATICS, (BIOSTEC 2016)
TI - Robustness to Sub-optimal Temperatures of the Processes of Tsr Cluster Formation and Positioning in Escherichia Coli
SN - 978-989-758-170-0
AU - Annila T.
AU - Neeli-Venkata R.
AU - Ribeiro A.
PY - 2016
SP - 137
EP - 141
DO - 10.5220/0005647001370141