Authors:
Emilie Bulard
1
;
Aurélie Bouchet-Spinelli
1
;
Patricia Chaud
2
;
André Roget
1
;
Roberto Calemczuk
1
;
Sébastien Fort
2
and
Thierry Livache
1
Affiliations:
1
Univ. Grenoble Alpes, CNRS and CEA, France
;
2
Univ. Grenoble Alpes and CNRS, France
Keyword(s):
Biosensor, Spri, Bacterial Detection, Carbohydrate, Escherichia Coli, Listeria Monocytogenes, Salmonella Enteritidis.
Related
Ontology
Subjects/Areas/Topics:
Biomedical Engineering
;
Biomedical Instrumentation
;
Biomedical Instruments and Devices
;
Biomedical Sensors
;
Emerging Technologies
;
Telecommunications
;
Wireless and Mobile Technologies
;
Wireless Information Networks and Systems
Abstract:
Food safety is a public health challenge. Devices allowing early, fast, label-free and in situ detection of
bacteria are of great interest to prevent outbreaks. Listeria monocytogenes, Salmonella spp. and Escherichia
coli O157:H7 are foodborne pathogens which were responsible of 60% of the hospitalizations in the USA in
2011. In this study, we conceived a carbohydrate microarray in order to detect and discriminate these three
food pathogenic bacteria. In less than 10 hours, from an initial bacterial suspension of 100 bacteria per mL,
Surface Plasmon Resonance imaging allowed the detection and the discrimination of these bacteria while
they were growing and interacting specifically with the carbohydrate microarray. Moreover, this device is
easily regenerable and can be re-used: it is probably a promising tool to early detect bacteria in food.