VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS

Victor Bertucci-Neto, Paulo Estevão Cruvinel

2011

Abstract

Turgor pressure is a physiologic variable of fundamental importance. It is a component of water potential and a measure of water status in a plant. For a long time direct measurement of turgor pressure was not possible. Three decades ago a pressure probe technique was originally introduced to measure turgor pressure and water relations of higher plant cells. Early experiments were made with a glass capillary linked to a pressure chamber, filled with silicone oil. After the vegetable cell to be punctured with the tip of the capillary, a sensor was used to measure the pressure in the chamber. From then until now the usual procedure has been to detect the meniscus position at the moment that the cell is punctured and manually, or automatically, to return the meniscus to the original position. When this occurs the pressure in the chamber is measured with a sensor. Some attempts were made to get the instrument automated but there is no systematic description about it. Based on this, it is proposed a dynamic model for the hydraulic system that can be helpful to design a closed loop system aiming an automated instrument. It is also shown that the theoretical model reasonably matches the experimental results.

References

  1. Bertucci-Neto, V. 2005. Modelagem e automação em nova técnica de medida para relações de água e planta. Thesis (in portuguese). Escola de Engenharia de São Carlos, Universidade de São Paulo. São Carlos.
  2. Bertucci-Neto, V. 2005. Modelagem e automação em nova técnica de medida para relações de água e planta. Thesis (in portuguese). Escola de Engenharia de São Carlos, Universidade de São Paulo. São Carlos.
  3. Cosgrove, D. J.; Durachko, D. M. 1986. Automated pressure probe for measurement of water transport properties of higher plant cells. Review of Scientific Instrument. v.57. n.10. p.2614-2619.
  4. Cosgrove, D. J.; Durachko, D. M. 1986. Automated pressure probe for measurement of water transport properties of higher plant cells. Review of Scientific Instrument. v.57. n.10. p.2614-2619.
  5. Doebelin, E. O. 1990. Measurement systems application and design. 4. ed. New York: McGraw-Hill Publishing Company.
  6. Doebelin, E. O. 1990. Measurement systems application and design. 4. ed. New York: McGraw-Hill Publishing Company.
  7. Husken, D.; Steudle, E.; Zimmermann, U. 1978. Pressure probe technique for measuring water relations of cells in higher plants. Plant Physiology. v. 61. p. 158-163.
  8. Husken, D.; Steudle, E.; Zimmermann, U. 1978. Pressure probe technique for measuring water relations of cells in higher plants. Plant Physiology. v. 61. p. 158-163.
  9. Steudle, E. 1993. Pressure probe technique: basic principles and application to studies of water and solute relations at the cell, tissue and organ level. Ed. J. A. C. Smith and H. Griffiths . Oxford, UK: Bios Scientific Publishers Ltd. p.5-36.
  10. Steudle, E. 1993. Pressure probe technique: basic principles and application to studies of water and solute relations at the cell, tissue and organ level. Ed. J. A. C. Smith and H. Griffiths . Oxford, UK: Bios Scientific Publishers Ltd. p.5-36.
  11. Vennard, J. K. (1961). Elementary fluid dynamics. 4. ed. New York: John Wiley and Sons, Inc.
  12. Vennard, J. K. (1961). Elementary fluid dynamics. 4. ed. New York: John Wiley and Sons, Inc.
Download


Paper Citation


in Harvard Style

Bertucci-Neto V. and Estevão Cruvinel P. (2011). VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS . In Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO, ISBN 978-989-8425-74-4, pages 433-436. DOI: 10.5220/0003539704330436


in Harvard Style

Bertucci-Neto V. and Estevão Cruvinel P. (2011). VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS . In Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO, ISBN 978-989-8425-74-4, pages 433-436. DOI: 10.5220/0003539704330436


in Bibtex Style

@conference{icinco11,
author={Victor Bertucci-Neto and Paulo Estevão Cruvinel},
title={VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS},
booktitle={Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO,},
year={2011},
pages={433-436},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003539704330436},
isbn={978-989-8425-74-4},
}


in Bibtex Style

@conference{icinco11,
author={Victor Bertucci-Neto and Paulo Estevão Cruvinel},
title={VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS},
booktitle={Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO,},
year={2011},
pages={433-436},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0003539704330436},
isbn={978-989-8425-74-4},
}


in EndNote Style

TY - CONF
JO - Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO,
TI - VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS
SN - 978-989-8425-74-4
AU - Bertucci-Neto V.
AU - Estevão Cruvinel P.
PY - 2011
SP - 433
EP - 436
DO - 10.5220/0003539704330436


in EndNote Style

TY - CONF
JO - Proceedings of the 8th International Conference on Informatics in Control, Automation and Robotics - Volume 1: ICINCO,
TI - VALIDATED MODEL OF A PRESSURE MICROPROBE FOR WATER RELATIONS OF PLANT CELLS
SN - 978-989-8425-74-4
AU - Bertucci-Neto V.
AU - Estevão Cruvinel P.
PY - 2011
SP - 433
EP - 436
DO - 10.5220/0003539704330436