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Author: Silvia Mittler

Affiliation: University of Western Ontario, Canada

ISBN: 978-989-758-174-8

Keyword(s): Waveguide, Evanescent Illumination, Microscopy, WEEF and WEFS, Cells, Bacteria, Fluorescence, Scattering, Distance Mapping, Granularity, Application as a Sensor.

Related Ontology Subjects/Areas/Topics: Photodetectors, Sensors and Imaging ; Photonics ; Photonics, Optics and Laser Technology

Abstract: Two evanescent field microscopy technologies based on glass slab waveguides with permanent coupling gratings are presented: waveguide evanescent field fluorescence microscopy (WEFF) and waveguide evanescent field scattering microscopy (WEFS). The technologies are briefly described: the experimental setup is based on a conventional inverted microscope. A comparison to TIR and TIRF microscopy is given. The advantages of the waveguide method are clearly addressed. Various examples from for WEFF and WEFS microscopy are given. For WEFF: static distance mapping with a multimode waveguide, dynamic solubilisation studies of cell plasma membranes and the kinetic response of osteoblasts to trypsin. For WEFS: bacteria sterilization as well as cell adhesion and granularity studies. The latest development is a mass producible all-polymer-waveguide-chip to bring the technology to the interested scientific community.

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Paper citation in several formats:
Mittler S. (2016). Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics.In Proceedings of the 4th International Conference on Photonics, Optics and Laser TechnologyISBN 978-989-758-174-8, pages 201-212. DOI: 10.5220/0005618302010212

@conference{photoptics16,
author={Silvia Mittler},
title={Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics},
booktitle={Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology},
year={2016},
pages={201-212},
doi={10.5220/0005618302010212},
isbn={978-989-758-174-8},
}

TY - CONF

JO - Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology
TI - Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics
SN - 978-989-758-174-8
AU - Mittler S.
PY - 2016
SP - 201
EP - 212
DO - 10.5220/0005618302010212

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